- Other Names
- CD121A, D2S1473, P80, IL-1R alpha
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- Product Citations
Human IL-1R was initially cloned from T cells and fibroblasts. It belongs to the family of IL-1 like receptors that, in conjuction with Toll-like receptors, constitute a phylogenetically conserved family of proteins. A characteristic of this family is the presence of a TIR domain (Toll/IL-1R domain) in the cytoplasmic portion. IL-1R contains a large cytoplasmic region, and the extracellular region consists of three immunoglobulin-like domains. After IL-1 binds to IL-1R1, IL-1R accessory protein (AcP) is recruited to form a high affinity IL-1R1-IL-1RAcP heterodimeric receptor. Subsequently, the IL-1RI-IL-1RAcP complex recruits the myeloid differentiation protein (MyD88), IRAKs, and TNF receptor associated factor 6 (TRAF6). The downstream signaling cascade involves activation of NFκB, mitogen-activated protein kinase (MAPK), and c-Jun N-terminal kinase (JNK) pathways. Soluble IL-1RI and IL-1RII bind to IL-1β with higher affinity than do IL-1α and IL-1Ra, preventing IL-1RI signaling. IL-1 is induced and activated after tissue injury, and IL-1/IL-1R play an essential role in inflammatory conditions such as sepsis, rheumatoid arthritis, inflammatory bowel disease, keratinocyte transformation, and carcinogenesis.Product Details
- Human IL-1Ra, amino acids (Asp21-Thr332) (Accession# M27492) was expressed in 293E cells.
- Molecular Mass
- The 333 amino acid recombinant protein has a predicted molecular mass of approximately 38 kD. The DTT-reduced and non-reduced protein migrate at approximately 55-60 kD by SDS-PAGE. The N-terminal contains 9H-2(SGGG)-IEGR-tag.
- >95%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.) Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HSA. After dilution, the cytokine can be stored between 2°C and 8°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
- ED50 = 0.2 - 0.6 µg/ml, corresponding to a specific activity of 1.7 - 5.0 x 103 units/mg, as determined by the dose dependent inhibition of D10.G4.1 cell proliferation.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at email@example.com.
T cells, fibroblasts, endothelial cells, epithelial cells, monocytes, dendritic cells, cardiomyocytes
- IL-1R plays a key role in the innate and adaptive immune response. It is the receptor for IL-1α and IL-1β. IL-1/IL-1R complex induces the expression of inflammatory cytokines, adhesion molecules, colony-stimulating factors, and mesenchymal growth factor. IL-1R also intervenes in the differentiation and commitment of Th17 cells. IL-1R signaling is regulated by IL-1Ra (antagonist), IL-1RII (decoy receptor), TIR8 (intracellular inhibitor), soluble IL-1RI and IL-1RII.
- IL-1α, IL-1β, IL-1R antagonist
- Biology Area
- Cell Biology, Signal Transduction, Immunology
- Molecular Family
- Soluble Receptors, Cytokine/Chemokine Receptors
- Antigen References
1. Sims JE, et al. 1989. PNAS 86:8946.
2. Luheshi NM, et al. 2009. Br. J. Pharmacol. 157:1318.
3. Barlo NP, et al. 2011. Clin. Exp. Immunol. 166:346.
4. Riva F, et al. 2012. Front. Immunol. 3:322.
5. Cataisson C, et al. 2012. J. Exp. Med. 209:1689.
6. van de Veerdonk FL and Netea MG. 2013. Front. Immunol. 4:167.
7. Garlanda C, et al. 2013. Front. Immunol. 4:180.
8. Vecile E, et al. 2013. PLoS One 8:e53265.
- Gene ID
- 3554 View all products for this Gene ID
- View information about IL-1R on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.