Recombinant Human GDNF (Animal-Free)

Pricing & Availability
Other Names
Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor (ATF)
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711002 10 µg 172€
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Description

Glial cell line-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily and was first identified as a growth factor supporting the survival of embryonic midbrain neurons. GDNF has been shown to promote the development, differentiation, and survival of dopaminergic neurons by signaling through GDNF family receptor α-1 (GFRα1) and the coreceptor Ret receptor tyrosine kinase. Dysregulated expression of GDNF has been reported to be associated with several mental diseases, including depression and schizophrenia. GDNF is considered as a promising therapeutic agent for the treatment of Parkinson's disease, since GNDF can protect dopaminergic neurons from neurotoxin and promotes neuron regeneration after injury. In addition to the functions in the nervous system, GDNF is also required for the differentiation of spermatogonia and development of kidney. Upregulated expression of GNDF was observed in various cancer cell lines and correlates with malignant cancer progression, suggesting a role of GDNF in carcinogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human GDNF, amino acids (Ser78 - Ile211) (Accession# NP_000505.1) with an N-terminal Met, was expressed in E. coli.
Molecular Mass
The 135 amino acid recombinant protein has a predicted molecular mass of approximately 15.0 kD. Recombinant human GDNF forms a a disulfide-linked homodimer. The predicted N-terminal amino acid is Met.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
Lyophilized, carrier-free.
Endotoxin Level
Less than 0.1 ng per µg of protein.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store in working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
ED50 ≤0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg, as measured by its ability to stimulate proliferation of rat C6 cells in a dose dependent manner.
Application

Bioassay

Application Notes

This product is reactive with human, mouse, and monkey.

Antigen Details

Structure
Growth factor
Distribution
GDNF is a secreted protein and is widely expressed in the developing and adult central nervous system.
Function
GDNF is a neurotrophic factor, promoting the development, differentiation, and survival of dopaminergic neurons. GNDF participates in the recovery processes after brain injury.
Interaction
GDNF targets the neuronal cells expressing GDNF family receptors.
Ligand/Receptor
GDNF interacts with GDNF family receptor α-1 (GFRα1). Lower affinity with GFRα2, GFRα3, and GFRα4.
Bioactivity
Recombinant human GDNF is able to induce proliferation of rat C6 cells.
Cell Type
Neural Stem Cells, Embryonic Stem Cells
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Allen SJ, et al. 2013. Pharmacol. Ther. 138:155.
2. Hendrich J, et al. 2012. Neuroscience. 219:204.
3. Hidalgo-Figueroa M, et al. 2012. J. Neurosci. 32:864.
4. Pascual A, et al. 2011. J. Mol. Endocrinol. 46:83.
5. Carnicella S, et al. 2009. Pharmacol. Ther. 122:9.
6. Sariola H, et al. 2003. J. Cell. Sci. 116:3855.

Gene ID
2668 View all products for this Gene ID
UniProt
View information about GDNF on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 3    Revision Date: 05-06-2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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