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Ultra-LEAF™ Purified anti-human VISTA (PD-1H) Antibody

Pricing & Availability
Clone
W20178L (See other available formats)
Regulatory Status
RUO
Other Names
V-Set Immunoregulatory Receptor (VSIR), V-Domain Ig Suppressor of T Cell Activation (VISTA), Stress-Induced Secreted Protein-1 (SISP1), PDCD1 Homolog, Platelet Receptor GI24 (GI24), Death Domain1alpha, DD1 alpha, PP2135, C10orf54, DD1alpha, B7-H5
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20178L_ULEAF_VISTA_Antibody_100422
Biotinylated recombinant human VISTA-Avi (black circles) binds to immobilized recombinant human IGSF11/BT-IgSF/VSIG3-FC. Ultra-LEAF™ purified anti-human VISTA (PD-1H) (clone W20178L) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have the effect. This antibody blocks the binding of 5 µg/mL biotinylated recombinant human VISTA-Avi to 2 µg/mL immobilized recombinant human IGSF11/BT-IgSF /VSIG3-FC. ND50 range: 1-6 µg/mL.
  • W20178L_ULEAF_VISTA_Antibody_100422
    Biotinylated recombinant human VISTA-Avi (black circles) binds to immobilized recombinant human IGSF11/BT-IgSF/VSIG3-FC. Ultra-LEAF™ purified anti-human VISTA (PD-1H) (clone W20178L) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have the effect. This antibody blocks the binding of 5 µg/mL biotinylated recombinant human VISTA-Avi to 2 µg/mL immobilized recombinant human IGSF11/BT-IgSF /VSIG3-FC. ND50 range: 1-6 µg/mL.
Cat # Size Price Quantity Check Availability Save
607353 100 µg 260€
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607354 1 mg 964€
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Description

VISTA, V-domain Ig suppressor of T cell activation, suppresses T cell activation and was initially identified by affymetrix analysis of activated versus resting mouse CD25+ CD4+ natural Treg cells. Human Vista is a type I transmembrane protein with a single extracellular IgV domain. It is 311 amino acids (aa) long, contains a 32-aa signal peptide, a 130-aa extracellular IgV domain, 33-aa stalk region, 20-aa transmembrane domain, and 96-aa cytoplasmic tail. VISTA shares significant sequence homology with the B7 family ligands PD-L1 and PD-L2. VISTA is predominantly expressed in hematopoietic tissues, highly expressed in human placenta, which may be indicative of a functional role for VISTA in allofetal tolerance. Flow cytometry shows that human VISTA is expressed in subsets of CD11bhi blood monocytes, dendritic cells, about 20% of CD4, with low density in CD8 T cells, and CD66b+ neutrophils at an intermediate intensity. VISTA serves both as a ligand (for antigen presenting cells) and as a receptor (for T cells). B7-H5 expressing on APCs or soluble B7-H5-Ig fusion proteins inhibit T cell proliferation and cytokine production in vitro. B7-H5 also inhibits the expression of T cell activation markers CD44 and CD69. In murine tumor models, anti-VISTA monoclonal antibodies (mAbs) prime to an increased number and elevated function of intratumoral T cells and in that way boost antitumor immunity. VISTA is negative checkpoint regulator and a promising target for cancer immunotherapy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human VISTA(PD-1H)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block – Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 5 µg/mL biotinylated recombinant human VISTA- Avi to 2 µg/mL immobilized recombinant human IGSF11/BT-IgSF/VSIG3-FC. ND50 range: 1-6 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. 

RRID
AB_2927926 (BioLegend Cat. No. 607353)
AB_2927926 (BioLegend Cat. No. 607354)

Antigen Details

Structure
Dimer
Distribution

Hematopoietic cells, monocytes, macrophages, dendritic cell, granulocytes, subsets of T cells

Function
VISTA suppresses proliferation of T cells and decreases their cytokine production and activation markers.
Interaction
Naïve T cells
Ligand/Receptor
Human recombinant VISTA inhibits the anti CD3-induced proliferation of activated human T cells.
Cell Type
Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
Soluble Receptors
Antigen References
  1. Wang Li, et al. 2011. J Exp Med. 208:577-92.
  2. Zhu Y, et al. 2013. Nat Commun. 4:2043.
  3. Ceeraz S, et al. 2013. Trends Immunol. 34:556-63.
  4. Le Mercier I, et al. 2014. Cancer Res. 74:1933-44.
  5. Lines JR, et al. 2014. Cancer Res. 74:1924-32.
  6. Flies DB, et al. 2014. J Clin Invest. 124:1966-75.
  7. Böger C, et al. 2017. Oncoimmunology. 6:e129321.
Gene ID
64115 View all products for this Gene ID
UniProt
View information about VISTA on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 10-04-2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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