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Ultra-LEAF™ Purified anti-SARS-CoV-2 S Protein S1 Antibody

Pricing & Availability
Clone
A20103J (See other available formats)
Regulatory Status
RUO
Other Names
Spike glycoprotein, S glycoprotein (E2)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A20103J_ULEAF_SARS-CoV-2-S_Protein-S1_Antibody_021521
Recombinant human ACE2 (Cat. No. 792002) (black circles) binds to immobilized recombinant SARS-CoV-2 S protein S1-Fc chimera (Cat. No. 793004) in a dose-dependent manner. Purified anti-SARS-CoV-2 S protein S1 antibody (clone A20103J) (purple squares) inhibits the binding in a dose-dependent manner. This antibody blocks the binding of 0.5 µg/mL recombinant human ACE2 to 1.0 µg/mL immobilized recombinant SARS-CoV-2 S protein S1-Fc chimera. ND50 range: 0.04 - 0.2 µg/mL.
  • A20103J_ULEAF_SARS-CoV-2-S_Protein-S1_Antibody_021521
    Recombinant human ACE2 (Cat. No. 792002) (black circles) binds to immobilized recombinant SARS-CoV-2 S protein S1-Fc chimera (Cat. No. 793004) in a dose-dependent manner. Purified anti-SARS-CoV-2 S protein S1 antibody (clone A20103J) (purple squares) inhibits the binding in a dose-dependent manner. This antibody blocks the binding of 0.5 µg/mL recombinant human ACE2 to 1.0 µg/mL immobilized recombinant SARS-CoV-2 S protein S1-Fc chimera. ND50 range: 0.04 - 0.2 µg/mL.
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944703 100 µg 287€
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944704 1 mg 964€
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Description

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single-stranded RNA virus that belongs in a family of viruses known as coronaviruses. SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and causes symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE2) expressed on host cells. This protein is composed of two subunits S1 and S2. S1 is responsible for binding to the host cell via ACE2 receptor and the S2 subunit is responsible for fusion with the host cell. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
SARS-CoV-2
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
SARS-CoV-2
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Blocking - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.5 µg/mL recombinant human ACE2 (Cat. No. 792002) to 1 µg/mL immobilized recombinant SARS-CoV-2 S protein S1-Fc chimera (Cat. No. 793004). ND50 range: 0.04 - 0.2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2890874 (BioLegend Cat. No. 944703)
AB_2890874 (BioLegend Cat. No. 944704)

Antigen Details

Structure
Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 a.a with a molecular weight of approximately 141 kD and consists of S1 and S2 subunits. S1 subunit is 673 a.a. long.
Distribution

Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane

Function
Host cell surface receptor binding
Interaction
ACE2
Ligand/Receptor
ACE2
Biology Area
COVID-19
Antigen References

1. Walls AC, et al. 2020. Cell. 181(2):281-292.
2. Yan R, et al. 2020. Science. 367 (6485):1444-1448.
3. Wrapp D, et al. 2020. Science. 367 (6483):1260-1263.
4. Shang J, et al. 2020. PNAS. 117(21):11727-11734

Gene ID
43740568 View all products for this Gene ID
UniProt
View information about Viral Protein on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02-15-2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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