Ultra-LEAF™ Purified anti-mouse/human LAP (TGF-β1) Antibody

Pricing & Availability
Clone
TW7-28G11 (See other available formats)
Other Names
TGFB, TGFB1, DPD1, Latency Associated Peptide (LAP), TGF-β1, TGF-β
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
TW7-28G11_LEAF_LAP_Antibody_FC_102813.jpg
C57BL/6 mouse platelets were stained with LEAF™ purified anti-LAP (TGF-β1, clone TW7-28G11) (filled histogram) or LEAF™ purified mouse IgG2b, κ (open histogram), followed by anti-mouse IgG2b-PE.
  • TW7-28G11_LEAF_LAP_Antibody_FC_102813.jpg
    C57BL/6 mouse platelets were stained with LEAF™ purified anti-LAP (TGF-β1, clone TW7-28G11) (filled histogram) or LEAF™ purified mouse IgG2b, κ (open histogram), followed by anti-mouse IgG2b-PE.
Cat # Size Price Quantity Avail. Save
146703 100 µg 124€
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146704 1 mg 468€
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Description

Transforming growth factor beta (TGF-β) is a cytokine that has critical functions in the immune response by regulating Treg and Th17 cell differentiation. TGF-β is first synthesized as pro-TGF-β which is then cleaved by furin proprotein convertase in the Golgi apparatus to produce a latent TGF-β complex. This complex consists of non-covalently associated dimers of the N-terminal region of pro-TGF-β (latency-associated peptide, LAP) and C-terminal region of pro-TGF-β (mature TGF-β). The latent-TGF-β has been found on the membrane of activated Treg cells, immature dendritic cells, megakaryocytes, and platelets.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Tgfb1'transduced P3U1 cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
WB, IP, Neut - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.2 TW7-28G11 recognizes conformation specific latent TGF-β complex (LAP + TGF-β) and pro-TGF-β1, but not free LAP or active TGF-β.

Additional reported applications (for relevant formats) include: Western blotting1, immunoprecipitation1, and neutralization2. TW7-28G11 is able to neutralize TGF-β activity.

Application References
  1. Oida T and Weiner HL. 2010. PloS ONE 5:e15523. (FC, IP, WB)
  2. Oida T and Weiner HL. 2011. PLoS ONE 6:e18365. (Neut)
RRID
AB_2562709 (BioLegend Cat. No. 146703)
AB_2562710 (BioLegend Cat. No. 146704)

Antigen Details

Structure
Dimers of latency-associated peptide non-covalently associated with dimers of mature TGF-β
Distribution
Many cell types, highly expressed on activated Treg cells and platelets
Function
TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response.
Ligand/Receptor
TGF-β receptors
Cell Type
Tregs, Platelets, Dendritic cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Oida T and Weiner HL. 2010. PLoS One 5:e15523.
2. Tran DQ, et al. 2009. Proc. Natl. Acad. Sci. USA 106:13445. 
3. Ochi H, et al. 2006. Nat. Med. 12:627.
4. Oida T, et al. 2003. J. Immunol. 170:2516. 
5. Nakamura K, 2001. J. Exp. Med. 194:629.
6. Miyazono K, et al. 1993. Growth Factor 8:11.

Gene ID
21803 View all products for this Gene ID
UniProt
View information about LAP on UniProt.org

Related FAQs

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 08-27-2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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