Ultra-LEAF™ Purified anti-human CD120b Antibody

Pricing & Availability
Clone
3G7A02 (See other available formats)
Regulatory Status
RUO
Other Names
TNFRSF1B, p75, p75TNFR, TNFR2, TNFR80, TBPII
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
3G7A02_CD120B_FC_ULEAF_021814.jpg
TNF-RII/TNFRSF1B inhibition of TNF-α-induced cytotoxicity and neutralization by human CD120b antibody. Recombinant Human sTNF-RII (Cat. No. 591602) inhibits Recombinant Human TNF-α (Cat. No. 570102) induced cytotoxicity in the L929 mouse fibroblast cell line in a dose-dependent manner (black line). Inhibition of Recombinant Human TNF-α (0.25 ng/mL) activity elicited by Recombinant Human sTNF-RII (0.4 µg/mL) is neutralized by increasing concentrations of human CD120b monoclonal antibody (red line) in the presence of the metabolic inhibitor actinomycin D (1 µg/ml). The ED50 is 0.5-1.5 µg/ml.
  • 3G7A02_CD120B_FC_ULEAF_021814.jpg
    TNF-RII/TNFRSF1B inhibition of TNF-α-induced cytotoxicity and neutralization by human CD120b antibody. Recombinant Human sTNF-RII (Cat. No. 591602) inhibits Recombinant Human TNF-α (Cat. No. 570102) induced cytotoxicity in the L929 mouse fibroblast cell line in a dose-dependent manner (black line). Inhibition of Recombinant Human TNF-α (0.25 ng/mL) activity elicited by Recombinant Human sTNF-RII (0.4 µg/mL) is neutralized by increasing concentrations of human CD120b monoclonal antibody (red line) in the presence of the metabolic inhibitor actinomycin D (1 µg/ml). The ED50 is 0.5-1.5 µg/ml.
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358408 500 µg 308€
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Description

CD120b, also known as TNFRII or TNFRSF1B, belongs to the TNF receptor superfamily and can interact with ligands TNF-α and TNF-β (also known as LT-α). It is a 75 kD type I transmembrane protein expressed on lymphocytes, monocytes, and granulocytes. When present in a heterocomplex with CD120a, it induces the recruitment of anti-apoptotic molecules, c-IAP1 and c-IAP2 that possess E3 ubiquitin ligase activity. Mutations in CD120b are associated with TNF-associated periodic syndrome (TRAPS) or periodic fever syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial CD120b recombinant protein expressed in 293E cells (22-211aa).
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC, Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralization. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Ye LL, et al. 2020. Annals of Translational Medicine. 8(24):1647. PubMed
  2. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
RRID
AB_2563224 (BioLegend Cat. No. 358408)

Antigen Details

Structure
75 kD, type I transmembrane protein, member of the TNF-receptor superfamily, forms a heterocomplex with TNFRI
Distribution

Expressed on cells of hematopoietic lineage including monocytes, lymphocytes, and granulocytes

Function
When complexed with TNFRI, recruits anti-apoptotic molecules c-IAP1 and c-IAP2 that possess ubiquitin ligase activity; cysteine rich extracellular region
Interaction
Interacts with TTRAP and TRAF2
Ligand/Receptor
TNF-α and TNF-β (LT-α)
Cell Type
Granulocytes, Lymphocytes, Monocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Transcription Factors
Molecular Family
CD Molecules
Antigen References

1. Andrews JS, et al. 1990. J. Immunol. 144:2582.
2. Nophar Y, et al. 1990. EMBO J. 9:3269.
3. Kohno T, et al. 1990. Proc. Natl. Acad. Sci. USA 87:8331.
4. Tartaglia LA, et al. 1991. Proc. Natl. Acad. Sci. USA 88:9292.
5. Hijdra D, et al. 2012. J. Inflamm. (Lond). 9:38.
6. Chen X, et al. 2013. J. Immunol. 190:1076.

Gene ID
7133 View all products for this Gene ID
UniProt
View information about CD120b on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02-24-2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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