TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody

Pricing & Availability
Clone
S17011E (See other available formats)
Regulatory Status
RUO
Other Names
CD16, CD32
Isotype
Rat IgG2b, κ
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Product Citations
publications
TruStain_FcX_Plus_CD16-CD32_Antibody_122018_updated.png
BALB/c splenocytes were incubated with TruStain FcX™ PLUS (clone S17011E, 0.25µg/106 cells, filled histogram) or TruStain FcX™ (clone 93, 1µg/106 cells, red line histogram) to block the Fc receptors, or were left untreated (black line histogram); then stained with CD90.2 (Thy-1.2, clone 53-2.1) FITC. Note the high background in the untreated cells and the bigger reduction in the background staining when the cells were incubated with TruStain FcX™ Plus compared to TruStain FcX™.
  • TruStain_FcX_Plus_CD16-CD32_Antibody_122018_updated.png
    BALB/c splenocytes were incubated with TruStain FcX™ PLUS (clone S17011E, 0.25µg/106 cells, filled histogram) or TruStain FcX™ (clone 93, 1µg/106 cells, red line histogram) to block the Fc receptors, or were left untreated (black line histogram); then stained with CD90.2 (Thy-1.2, clone 53-2.1) FITC. Note the high background in the untreated cells and the bigger reduction in the background staining when the cells were incubated with TruStain FcX™ Plus compared to TruStain FcX™.
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156603 50 µg 52€
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156604 500 µg 221€
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Description

CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ PLUS is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors and is more effective than TruStain FcX™.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Purity
The antibody was purified by affinity chromatography.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Concentration
0.5 mg/ml
Storage & Handling
The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ PLUS for 5-10 minutes, on ice, at 0.25 µg per 106 cells in a volume of 100 µl, prior to immunostaining. It is not necessary to wash the cells between the blocking and immunostaining steps.

Application Notes

Clone S17011E blocks both clone 93 and 2.4G2 also raised against mouse CD16/32

Product Citations
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  11. Kienzl M, et al. 2020. Oncoimmunology. 9:1776059. PubMed
  12. Westphal A, et al. 2017. J Exp Med. 214:227. PubMed
  13. Geng T, et al. 2022. Methods Mol Biol. 2585:71. PubMed
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  16. Thinard R, et al. 2022. Pharmaceutics. 14:. PubMed
  17. So EY, et al. 2021. Am J Physiol Cell Physiol. 321:C569. PubMed
  18. Wang JC, et al. 2022. Elife. 11:. PubMed
  19. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  20. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  21. Dong L, et al. 2022. Cells. 11:. PubMed
  22. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  23. Ejima R, et al. 2021. Nutrients. 13:. PubMed
  24. Guo S, et al. 2021. Sci Rep. 11:23745. PubMed
  25. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  26. Grubiši? V, et al. 2020. Cell Rep. 32:108100. PubMed
  27. Galani IE, et al. 2022. STAR Protoc. 3:101151. PubMed
  28. Olson B, et al. 2021. Brain Behav Immun. 97:102. PubMed
  29. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  30. Montel-Hagen A, et al. 2022. Curr Protoc. 2:e403. PubMed
  31. Chen AF, et al. 2022. Nat Methods. 19:547. PubMed
  32. Rocha-Resende C, et al. 2020. JCI Insight. 5:00. PubMed
  33. Kos S, et al. 2019. PLoS One. 14:e0217762. PubMed
  34. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  35. Chen S, et al. 2022. iScience. 25:105176. PubMed
  36. Oya Y, et al. 2021. Front Immunol. 12:779709. PubMed
  37. Hobbs BE, et al. 2021. Pathogens. 10:. PubMed
  38. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  39. Fan ZP, et al. 2021. Front Immunol. 12:743354. PubMed
  40. Phan TS, et al. 2021. Sci Adv. 7:. PubMed
  41. Casselli T, et al. 2021. PLoS Pathog. 17:e1009256. PubMed
  42. Jiang Y, et al. 2021. Nat Commun. 12:742. PubMed
  43. Madsen CS, et al. 2022. Commun Biol. 5:888. PubMed
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  48. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
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  52. Badgujar DC, et al. 2020. PLoS Pathog. 16:e1009016. PubMed
  53. Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed
RRID
AB_2783137 (BioLegend Cat. No. 156603)
AB_2783138 (BioLegend Cat. No. 156604)

Antigen Details

Structure
Ig superfamily, 40-60 kD
Distribution

B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells

Ligand/Receptor
IgG
Cell Type
B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14130 View all products for this Gene ID 14131 View all products for this Gene ID
UniProt
View information about CD16/32 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01-22-2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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