Recombinant Human LIF (ELISA Std.)

Pricing & Availability
Other Names
Leukemia Inhibitory Factor, HILDA (human interleukin in DA cells), MLPLI (melanoma-derived LPL inhibitor), DIA (differentiation-inducing factor)
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Human_LIF_Antibody_ELISA_011416
  • Human_LIF_Antibody_ELISA_011416
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593909 4 pack 64€
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Description

Human LIF stands for Leukemia Inhibitory Factor. It belongs to the IL-6 family of cytokines, and is a variably glycosylated, secreted protein with a mass of 38 – 67 kD. The mature form has 180 amino acids and contains six conserved cysteines, which form three intramolecular disulfide bonds. The protein itself forms a four-helix bundle fold. Human LIF shares 78% amino acid identity with mouse LIF.

LIF is a pleiotropic cytokine involved in cellular development, inflammation, immunology, and neoplasia. It often has contradictory effects within different contexts, either stimulating cell proliferation, or inducing differentiation of mouse M1 leukemic myeloid cell line into macrophages. With cultured embryonic stem cells, LIF has been an important laboratory tool to help maintain stem cell pluripotency for cell cultures. It is involved in the mechanisms of embryonic implantation, inflammatory disorders such as arthritis, immunomodulation, carcinogenesis, and neoplasia-associated cachexia.

LIF binds to both LIF Rα and gp130 on the cell surface to form a heterotrimeric complex, which initiates cell signaling through the JAK-STAT pathway. LIF can also activate the MAPK, PI3K, and p53 signaling pathways. Soluble forms of both binding partners do exist in circulation and potentially serve to further regulate LIF action.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human LIF, amino acids (Ser23-Phe202) (Accession# NP_002300.1), was expressed in E. coli.
Molecular Mass
The predicted molecular mass of human LIF is approximately 20 kD.
Purity
>97%, as determined by Coomassie stained SDS-PAGE.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Storage & Handling
Immediately upon receipt, store unopened vials between 2°C and 8°C and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial and vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes and then vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of two-fold dilutions from 1,000 to 15.6 pg/ml is suggested. It is recommended that each lot of the reagent be titrated for optimal performance of each application.

Application Notes

This LIF protein is useful as a standard for a human LIF sandwich ELISA, using purified anti-human LIF antibody (clone Poly5233) for capture and biotinylated anti-human LIF antibody (clone M017C3) for detection.

Antigen Details

Structure
Four-helix bundle fold.
Distribution

Expressed in the trophectoderm of the developing embryo and activated CD4+ T cells.

Function
LIF is a pleiotropic cytokine involved in cellular development, inflammation, immunology, and neoplasia.
Interaction
Embryonic stem cells.
Ligand/Receptor
LIF Rα, gp130
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Metcalf D. 1991. Int. J. Cell Cloning 2:95-108.
2. Zhang JG, et al. 1998. J. Biol. Chem. 17:10798.
3. Kamohara H, et al. 2007. Int. J. Oncol. 4:977.
4. Marshall MK, et al. 1994. Endocrinology 135:141.
5. Vogiagis D, Salamonsen LA. 1999. J. Endocrinol. 160:181.
6. Zouein FA, et al. 2013. Eur. Cytokine Netw. 24:11.
7. Mathieu ME, et al. 2012. Stem Cell Rev. 8:1.
8. Hu W, et al. 2007. Nature 450:721.
9. Trouillas M, et al. 2009. Eur. Cytokine Netw. 20:51.
10. Metcalfe SM. 2011. Genes Immun. 3:157.

Gene ID
3976 View all products for this Gene ID
UniProt
View information about LIF on UniProt.org

Related FAQs

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