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IL-17B is a disulfide-linked homodimer T cell-derived cytokine that belongs to the IL-17 family of structurally-related cytokines. They share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. This cytokine was reported to stimulate the release of TNF-α and IL-1β from a monocytic cell line. IL-17B is primarily localized to neuronal cell bodies. Numerous immune regulatory functions have been reported for the IL-17 family of cytokines, presumably due to their induction of many signaling molecules. The most notable role of IL-17 is its involvement in inducing and mediating proinflammatory responses. IL-17 is commonly associated with allergic responses. IL-17 induces the production of many other cytokines, such as IL-6, G-CSF, GM-CSF, IL-1β, TGF-β, TNF-α; chemokines including IL-8, GRO-α, and MCP-1; and prostaglandins from many cell types (fibroblasts, endothelial cells, epithelial cells, keratinocytes, and macrophages). The release of cytokines mediates several functions, such as airway remodeling, a characteristic of IL-17 responses. The increased expression of chemokines attracts other cells, including neutrophils but not eosinophils. IL-17 function is also essential to a subset of CD4+ T cells called Th17 cells. As a result of these roles, the IL-17 family has been linked to many immune/autoimmune related diseases including rheumatoid arthritis, asthma, lupus, allograft rejection, anti-tumor immunity and recently Psoriasis. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A and IL-17F can associate to form IL-17A/F heterodimer. IL-17B is expressed by T cells and has been shown to stimulate release of TNF-α and IL-1β from cells of monocyte lineage.Product Details
- Human IL-17B, amino acids (Gln21-Phe180) with an additional Met at the N-terminus (Accession# NP_055258.1), was expressed in E. coli.
- Molecular Mass
- The 161 amino acid recombinant protein has a predicted molecular mass of approximately 18.3 kD. Recombinant human IL-17B forms a non-disulfide-linked homodimer (36.6 kD). The predicted N-terminal amino acid is Met.
- >98%, as determined by Coomassie stained SDS-PAGE.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Human IL-17B is able to induce IL-8 secretion in human PBMCs using a concentration range of 10-100 ng/ml. Note: Results will vary with PBMC donors.
T cells, neurons
- Human IL-17B plays an important role in inflammatory responses, anti-tumor immunity, airway remodeling, and autoimmune diseases.
- Fibroblasts, endothelial cells, epithelial cells, keratinocytes, macrophages.
- Human IL-17B induces IL-8 secretion in PBMCs.
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Gene ID
- 27190 View all products for this Gene ID
- View information about IL-17B on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.