Recombinant Human CCL5 (RANTES) (ELISA Std.)

Pricing & Availability
Regulatory Status
RUO
Other Names
SIS-delta, T-cell specific protein p288, beta-chemokine RANTES, regulated upon activation, normally T-expressed, and presumably secreted, small inducible cytokine A5
Ave. Rating
Submit a Review
Product Citations
publications
CCL5_Human_Antibody_ELISA_122214
  • CCL5_Human_Antibody_ELISA_122214
Cat # Size Price Quantity Check Availability Save
580209 4 pack 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CCL5 (RANTES), also known as CCL5, is a 7.8 kD member of the CC family of chemokines, which is expressed in T cells and monocytes/macrophages. RANTES is strongly chemotactic for monocytes/macrophages, memory T cells, eosinophils, and induces histamine release from basophils. In a solution, RANTES can form high molecular weight aggregates that exhibit dissimilar biological activities to its dimeric form. The aggregates cause T cell and neutrophil activation while promoting HIV infection, whereas the dimeric form exhibits RANTES-induced chemotaxis and inhibits HIV infection. As T cells become activated, RANTES secretion appears to decrease.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human RANTES, amino acids (Ser24-Ser91) (Accession# NM_002985), was expressed in E. coli.
Molecular Mass
The 68 amino acid recombinant protein has a predicted molecular mass of approximately 7.851 kD. The DTT-reduced protein migrates at approximately 7 kD and the non-reduced protein migrates at approximately 9 kD by SDS-PAGE. The N-terminal amino acid is Ser.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Endotoxin Level
Less than 0.01ng/µg cytokine as determined by the LAL method.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
Application

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of two-fold dilutions from 500 pg/mL to 7.8 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This CCL5 protein is useful as a standard for a human CCL5 sandwich ELISA, using unlabeled J047C5 antibody (Cat. No. 526402) for capture and biotinylated Poly5197 antibody (Cat. No. 519703) for detection.

Note: For testing human CCL5 in serum, plasma or cell culture supernatant, LEGEND MAX™ Human CCL5 (RANTES) ELISA Kit with Pre-coated Plates (Cat. No. 440807 & 440808) are specially developed and recommended.

Antigen Details

Distribution

RANTES/CCL5 is secreted by lymphocytes, macrophages, eosinophils, platelets, and fibroblasts. RANTES is expressed by hepatocytes, sinusoidal endothelial cells and biliary epithelium during chronic hepatitis C.

Function
RANTES can promote IL-2 and IL-5 expression in human T cells, and the expression of IL-6 and IL-8 in fibroblasts from patients with RA. RANTES modulates the LPS-induced IL-6 and TNFα in peripheral blood monocytes; this modulation is through the induction of IL-10 by RANTES.
Interaction
RANTES/CCL5 is chemoattractant for memory and activated T cells, monocytes, dendritic cells, eosinophils, NK cells, mast cells, and basophils.
Ligand/Receptor
CCL5 binds to CCR1, CCR3 and CCR5 receptors. In addition CCL5 binds to CCR9; nevertheless, it does not induce calcium flux and therefore no chemotactic response.
Bioactivity
Bioactivity was measured by its property to chemoattract T cells in a dose dependent manner. The ED50 is 5-15 ng/ml.
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Brown KD, et al. 1989. J. Immunol. 142:679.
2. Schall TJ, et al. 1990. Nature 6294:669-671.
3. Cocchi F, et al. 1995. Science 270:1811-1815.
4. Shahrara S, et al. 2006. J. Immunol. 177:5077-5087.
5. Larrubia JR, et al. 2008. World J. Gastroenterol. 14:7149-7159.
6. Gleissner CA, et al. 2008. Arterioscler Thromb. Vasc. Biol. 28:1920-1927.
7. Von Luettichau, et al. 1996. Cytokine 8:89.
8. Schall TJ. 1991. Cytokine. 3:165.
9. Marques RE, et al. 2013. Expert Opin. Ther. Targets 17:1439.

Gene ID
6352 View all products for this Gene ID
UniProt
View information about CCL5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02-29-2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account