Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P)

Pricing & Availability
Clone
Poly18020 (See other available formats)
Regulatory Status
RUO
Other Names
CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, TUBB3, tubulin beta-4 chain, class III beta-tubulin
Previously
Covance Catalog# PRB-435P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
A_Poly18020_PURE_Tubulin_beta3_IHCP_1_032918
IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded rat cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A_Poly18020_PURE_Tubulin_beta3_IHCP_1_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded rat cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B_Poly18020_PURE_Tubulin_beta3_IHCP_2_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded human cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • C_Poly18020_PURE_Tubulin_beta3_IHCP_3_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded human cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly18020_PURE_Tubulin_beta3_WB_021915.jpg
    Western blot of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 0.1 µg/mL of Poly18020 or Rabbit IgG overnight at 4°C, followed by incubation with HRP-labeled anti-rabbit IgG (Cat. No. 410406). Enhanced chemiluminescence was used as the detection system.
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802001 100 µL 295,00€
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Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against microtubules derived from rat brain.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 0.5 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunocytochemistry (ICC).

This antibody is well characterized and highly reactive to neuron specific Class III ß-Tubulin (ßIII), but does not identify ß-tubulin found in glial cells. This product may contain other non-IgG subtypes.

Application References
  1. Feuer R, et al. 2005. J. Neurosci. 25(9):2434-44.
  2. Mozzetti S, et al. 2005. Clin. Cancer Res. 11(1):298-305.
  3. Feuer R, et al. 2003. Am. J. Pathol. 163(4):1379-93.
  4. Zhang XM, Yang XJ. 2001. Development. 128(6):943-57.
  5. Ma M, et al. 2012. Neurobiol Dis. 56:34. (WB) PubMed
  6. Tseung G, et al. 2011. J. Virol. 85:5718. (IF) PubMed
  7. Shimada IS, et al. 2012. J Neurosci. 32:7926. (IHC) PubMed
  8. Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF) PubMed
Product Citations
  1. Macpherson P, et al. 2015. Development. 142: 4038 - 4048. PubMed
  2. Ferguson M, et al. 2013. Neurobiol Dis. 56:34-46. PubMed
  3. Tsueng G, et al. 2011. J Virol. 85:5718-5732. PubMed
  4. Flores-Otero J, et al. 2007. J Neurosci. 27:14023-14034. PubMed
  5. Shimada I, et al. 2012. J Neurosci. 32:7926-7940. PubMed
  6. Chow A, Morshead C 2016. Stem Cell Res. 16:79-87. PubMed
  7. Yau K, et al. 2016. J Neurosci. 36: 1071 - 1085. PubMed
  8. Cui Q, et al. 2016. Nat Commun. 7:10637. PubMed
  9. Vicidomini C, et al. 2016. Mol Psychiatry. 10.1038/mp.2016.30. PubMed
  10. Otani T, et al. 2016. Cell Stem Cell. 18: 467-480. PubMed
  11. Anastasiadou S, Knöll B 2016. Exp Neurol. 279:243-260. PubMed
  12. Son M, et al. 2016. Exp Mol Med. 48: 232. PubMed
  13. Baleriola J, et al. 2016. Sci Rep. 6: 25928. PubMed
  14. Krishnan V, et al. 2016. J Neuropathol Exp Neurol. 75: 464 - 478. PubMed
  15. Tian E, et al. 2016. Cell Rep. 16: 781-792. PubMed
  16. Lin P, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G412 - G422. PubMed
  17. Zhang L, et al. 2016. Neuroscience. 337:88-97. PubMed
  18. Watanabe S, et al. 2016. EMBO Mol Med. 8(12):1421-1437. PubMed
  19. Brykczynska U, et al. 2016. Stem Cell Reports. 7:1059-1071. PubMed
  20. Boland M, et al. 2017. Brain. 10.1093/brain/aww357. PubMed
  21. Arber C, et al. 2020. Mol Psychiatry. 25:2919. PubMed
  22. Avraham O, et al. 2020. Nat Commun. 3.854861111. PubMed
  23. Bhaskaran S, et al. 2020. Aging Cell. 19:e13225. PubMed
  24. Ward AI, et al. 2020. MBio. 11:00. PubMed
  25. Nichterwitz S, et al. 2020. Genome Res. 30:1083. PubMed
  26. Omichi R, et al. 2020. Mol Ther Methods Clin Dev. 1.51875. PubMed
  27. Bosze B, et al. 2020. J Neurosci. 40:1501. PubMed
  28. Kurihara Y, et al. 2020. Journal of Neurochemistry. 155(3):285-299. PubMed
  29. Cates K, et al. 2020. Cell Stem Cell. 28(1):127-140.e9. PubMed
  30. Huang S, et al. 2020. Cell. 184(2):441-459.e25. PubMed
  31. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  32. Arber C, et al. 2021. Cell Reports. 34(2):108615. PubMed
  33. Gong C, et al. 2021. Cell Reports. 34(12):108889. PubMed
  34. Smith-Anttila CJA, et al. 2021. Developmental Biology. 476:18-32. PubMed
  35. Hung C, et al. 2021. Cell Reports. 35(11):109259. PubMed
  36. Ou MY, et al. 2021. Cell Reports. 35(13):109290. PubMed
  37. Tseng K, et al. 2017. eNeuro. 10.1523/ENEURO.0214-17.2017. PubMed
  38. Benoy V, et al. 2018. Brain. 10.1093/brain/awx375. PubMed
  39. Xie Y, et al. 2018. Stem Cell Res. 9:2010. PubMed
  40. Carlin D et al. 2018. eNeuro. 5(2) pii: ENEURO. PubMed
  41. Qiang L et al. 2018. Current biology : CB. 28(13):2181-2189 . PubMed
  42. Zahr SK et al. 2018. Neuron. 97(3):520-537 . PubMed
  43. Ou J et al. 2018. Cell. 173(4):851-863 . PubMed
  44. Kawakami Y et al. 2018. The Journal of Neuroscience. 38(10):2589-2604 . PubMed
  45. Jean-Louis T, et al. 2018. Neurobiol Aging. 62:130. PubMed
  46. Hung COY et al. 2018. Cell reports. 25(13):3647-3660 . PubMed
  47. Toda T, et al. 2017. Cell Stem Cell. 21:618. PubMed
  48. Makar T, et al. 2018. Stem Cell Res Ther. 9:127. PubMed
  49. Shang Y, et al. 2018. J Neurosci. 38:4006. PubMed
  50. Jeon H, et al. 2018. J Clin Med. 7:. PubMed
  51. Lee J, et al. 2018. J Clin Med. 7:. PubMed
  52. Stokowska A, et al. 2017. Brain. 140:353. PubMed
  53. Li H, et al. 2018. Hum Mutat. 39:550. PubMed
  54. Bowles KR, et al. 2019. PLoS One. 14:e0213374. PubMed
  55. Cora V, et al. 2019. Cells. 8:5. PubMed
  56. Meijer M et al. 2019. Cell Rep. 27(7):2199-2211 . PubMed
  57. Rhee HJ et al. 2019. Cell Rep. 27(7):2212-2228 . PubMed
  58. Carlin D et al. 2019. eNeuro. 6(3) pii: ENEURO. PubMed
  59. Hsu J, et al. 2019. Nat Commun. 10:2939. PubMed
  60. Mickle AD, et al. 2019. Nature. 565:361. PubMed
  61. Shah K, et al. 2019. J Mol Cell Biol. N/A. PubMed
  62. Uezono N, et al. 2018. Stem Cells. 36:737. PubMed
  63. Hoffman LF, et al. 2018. J Assoc Res Otolaryngol. 19:33. PubMed
  64. Sakaguchi H, et al. 2019. Stem Cell Reports. 13:458. PubMed
  65. Li H, et al. 2019. iScience. 19:559. PubMed
  66. Chabrat A, et al. 2019. Stem Cells Int. 2019:2945435. PubMed
  67. Tang SC, et al. 2017. Diabetologia. 61:158. PubMed
  68. Tiwari D et al. 2019. Neurobiol Dis. 1871:48:00 . PubMed
  69. Chanda S et al. 2019. Cell Stem Cell. 25(1):103-119 . PubMed
  70. Martínez JC et al. 2019. Neuron. 104(5):931-946 . PubMed
  71. Sar Shalom H, et al. 2019. Elife. 8:e41162. PubMed
  72. Voronova A et al. 2017. Neuron. 94(3):500-516 . PubMed
  73. Kim H et al. 2017. Stem cell research. 26:72-75 . PubMed
  74. Jiang Y, et al. 2018. J Biol Chem. 293:19317. PubMed
  75. Duncan DS, et al. 2018. Front Neurosci. 0.9875. PubMed
  76. Yang C, et al. 2020. Neuron. 105:276. PubMed
  77. Alvarez–Lindo N, et al. 2019. Sci Rep. 9:18486. PubMed
  78. Iannielli A, et al. 2019. Cell Rep. 29:4646. PubMed
  79. Suter TACS, et al. 2020. Cell Rep. 30:1164. PubMed
  80. Hattori Y, et al. 2020. Nat Commun. 11:1631. PubMed
  81. Arber C, et al. 2019. Brain Commun. 1:fcz024. PubMed
  82. Barry DM, et al. 2020. Nat Commun. 11:1397. PubMed
  83. Facchin C, et al. 2020. Theranostics. 10:3518. PubMed
  84. Brosig A, et al. 2019. Cell Rep. 29:2028. PubMed
  85. Sabelström H, et al. 2019. Cell Rep. 28:2064. PubMed
  86. Verstraelen P, et al. 2017. Front Cell Neurosci. 11:173. PubMed
  87. MOSES C, et al. 2020. Sci Rep. 10:11393. PubMed
  88. Hastings RL, et al. 2020. Sci Rep. 10:11132. PubMed
  89. Nayak G, et al. 2020. Cell Reports. 30(3):672-686.e8.. PubMed
  90. Grove M, et al. 2020. eLife. 9:e50138.. PubMed
  91. Girouard MP, et al. 2020. eneuro. 7(2):ENEURO. PubMed
  92. Peitz M, et al. 2020. STAR Protoc. 1:100038. PubMed
RRID
AB_2564645 (BioLegend Cat. No. 802001)

Antigen Details

Cell Type
Mature Neurons, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Gene ID
10381 View all products for this Gene ID
UniProt
View information about Tubulin beta-3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 10-24-2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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