- SYK-01 (See other available formats)
- Regulatory Status
- Other Names
- Spleen tyrosine kinase, protein tyrosine kinase SYK
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites. The SYK-01 monoclonal antibody (raised against amino acids 5-360 of human Syk) recognizes human, mouse, and rat and Syk and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.Product Details
- Verified Reactivity
- Human, Rat, Mouse
- Antibody Type
- Host Species
- Partial recombinant (amino acids 5-360) human Syk
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IHC-P - Verified
IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 0.5 µg per ml. For IHC, use a 10 µg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation.
This clone may produce a non-specific band of roughly ~250 kD at higher antibody concentrations
This clone does not recognize ZAP70
This clone has been reported to recognize rat Syk
- Application References
- Arudchandran R, et al. 2000. J. Exp. Med. 191:47. (IP WB)
- Swann PG, et al. 1999. J. Biol. Chem. 33:23068. (IP)
- Inoue M, et al. 2011. J. Immunol. 186:18. PubMed
- Hatterer E, et al. 2011. Neurosci Res. 70:172. PubMed
- Cunningham CC, et al. 2012. Clin Immunol. 144:228. PubMed
- Alsadeg A, et al. 2014. J Immunol. 193:268. PubMed
- Product Citations
AB_493087 (BioLegend Cat. No. 626201)
AB_2200268 (BioLegend Cat. No. 626202)
- Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
Cytoplasmic protein, widely expressed in hematopoietic cells
- Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
- c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, paxillin, FAK, Grb2, CD3ε, LAT, many others
- Phosphorylation (Y131, Y323, Y348, Y352, Y525, Y526)
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790.
2. Toyabe S-L, et al. 2001.Immunology 103:164.
- Gene ID
- 6850 View all products for this Gene ID
- View information about Syk on UniProt.org
Related Pages & Pathways
Other FormatsView All Syk Reagents Request Custom Conjugation
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.