Purified anti-PKC Antibody

Pricing & Availability
Clone
PKC0103 (See other available formats)
Other Names
Protein kinase C
Isotype
Mouse IgG2a, κ
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Product Citations
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PKC103_PURE_PKC_Antibody_1_WB_083016
Total cell lysates (15 µg protein) from HeLa, Jurkat, and Raw264.7 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-PKC antibody (clone PKC0103). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-β-actin antibody was used as a loading control.
  • PKC103_PURE_PKC_Antibody_1_WB_083016
    Total cell lysates (15 µg protein) from HeLa, Jurkat, and Raw264.7 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-PKC antibody (clone PKC0103). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-β-actin antibody was used as a loading control.
Cat # Size Price Quantity Avail. Save
624302 500 µl (20 Western blots) 200€
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Description

PKC, also known as protein kinase C, belongs to a kinase family involved in intracellular signaling processes. PKC isoforms are divided into three groups known as conventional (including PKCα, PKCβ1, PKCβ2 and PKCγ), novel (including PKCε, μ, θ, δ, η), and atypical PKC isoforms (such as ξ). Conventional PKC isoforms require calcium and diacylglycerol for activation, novel isoforms require diacylglycerol, and atypical isoforms require neither calcium nor diacylglycerol. Once activated by phosphorylation, the PKCs phosphorylate a large variety of substrates involved in cell differentiation, proliferation, and activation. The PKC0103 antibody recognizes human, mouse, and rat PKC and is useful for Western blotting and immunoprecipitation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Protein kinase C from bovine brain
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.
Preparation
This antibody was purified by affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 25 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone PKC0103 recognizes PKC α, β, and γ.

RRID
AB_315982 (BioLegend Cat. No. 624302)

Antigen Details

Structure
Serine/threonine kinase containing a regulatory and catalytic domain composed of a number of conserved regions interspersed with variable regions. Human PKCα has a predicted MW of 77kD; PKCβ has two isoforms with a predicted MW of 76.8kD and 77kD; PKCγ has two isoforms with a predicted MW of 78kD and 62kD.
Distribution

High expression in brain, isoforms vary in tissue expression

Function
PKC isoforms are involved in a variety of intracellular signaling processes mediated through phospholipids hydrolysis. Once activated by phosphorylation, the PKCs phosphorylate a large variety of substrates involved in cell differentiation, proliferation,
Modification
Phosphorylation
Biology Area
Cell Biology
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Liu WS, et al. 1998. Cell Signal 10:529.
2. Way KJ, et al. 2000. Trends Pharmacol. Sci. 21:181.

Gene ID
5578 View all products for this Gene ID 5579 View all products for this Gene ID 5582 View all products for this Gene ID
UniProt
View information about PKC on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 06-19-2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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