- W18054D (See other available formats)
- Other Names
- POU domain, class 2, transcription factor 1, Octamer-binding transcription factor 1, NF-A1
- Rat IgG2a, κ
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- Product Citations
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Oct-1 is a member of the Pit-Oct-Unc (POU) domain family of transcription factors, which are characterized by a bipartite POU domain. The POU domain that contains two DNA-binding subdomains; an N-terminal POU-specific subdomain and a C-terminal homeobox subdomain, each of which are tethered by a variable linker sequence. Oct-1 can function as both a transcriptional activator and repressor, and controls the expression of housekeeping genes such as those encoding Histone H2B and snRNPs, as well as genes involved in development and differentiation. The highly-modular POU domain enables Oct-1 to drive diverse transcriptional programming that occurs in a tissue-specific manner. The POU domains confers the flexibility for Oct-1 to function as both a monomer, dimer, and heterodimer, and also facilitates the interaction between Oct-1 with a broad set of transcriptional cofactor proteins. Moreover, Oct-1 undergoes multiple post-translational modifications that confers DNA-binding specificity to certain promoters, including phosphorylation, ubiquitination, and sumoylation. Oct-1 is highly-expressed in many types of cancers, and functionally different isoforms produced from alternative promoter usage have been reported.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Partial recombinant protein corresponding to human OCT-1 amino acid residues 251-500
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IP - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone was not tested for ICC
Non-specific band present in Ramos at 60 kD
- Oct-1 is a 743 amino acid protein with a predicted molecular weight of 76kD
- Transcription factor
- Biology Area
- Cell Biology, Transcription Factors
- Antigen References
- Pankratova EV, et al. 2018. Oncotarget. 9:29892
- Strum RA and Herr W. et al. 1988. Nature. 336:601.
- Strum RA, et al. 1988. Genes Dev. 2:1582.
- Gene ID
- 5451 View all products for this Gene ID
- View information about Oct-1 on UniProt.org
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