Purified anti-mouse CD3ε Antibody

Pricing & Availability
Clone
KT3.1.1 (See other available formats)
Other Names
CD3e, T3e
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
KT3-1-1_PURE_CD3e_Antibody_041918
C57 splenocytes were stained with purified CD3ε (clone KT3.1.1) (filled histogram) or rat IgG2a, κ isotype control (open histogram).
  • KT3-1-1_PURE_CD3e_Antibody_041918
    C57 splenocytes were stained with purified CD3ε (clone KT3.1.1) (filled histogram) or rat IgG2a, κ isotype control (open histogram).
  • KT3-1-1_PURE_CD3e_Antibody_050918
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-mouse CD3ε (clone KT3.1.1) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 647 Goat anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured by 10X objective.
Cat # Size Price Quantity Avail. Save
155602 500 µg 188€
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CBAT6 Thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Tomonari K. 1988. Immunogenetics. 28:455. (FC)
RRID
AB_2734240 (BioLegend Cat. No. 155602)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation
Ligand/Receptor
TCR
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References
  1. Preston S, et al. 1997. Eur. J. Immunol. 27:1911.
  2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
  3. Weiss A, Littman DR. 1994. Cell. 76:263.
Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3e on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05-09-2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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