- Clone
- KT3.1.1 (See other available formats)
- Other Names
- CD3e, T3e
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57 splenocytes were stained with purified CD3ε (clone KT3.1.1) (filled histogram) or rat IgG2a, κ isotype control (open histogram). -
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-mouse CD3ε (clone KT3.1.1) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 647 Goat anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured by 10X objective.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 155602 | 500 µg | 188€ | ||||
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CBAT6 Thymocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Validated - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
-
- Tomonari K. 1988. Immunogenetics. 28:455. (FC)
- RRID
-
AB_2734240 (BioLegend Cat. No. 155602)
Antigen Details
- Structure
- Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
- Distribution
-
Thymocytes (differentiation dependent), mature T cells, NK-T cells
- Function
- TCR signal transduction, T cell activation
- Ligand/Receptor
- TCR
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
- Preston S, et al. 1997. Eur. J. Immunol. 27:1911.
- Davis MM. 1990. Annu. Rev. Biochem. 59:475.
- Weiss A, Littman DR. 1994. Cell. 76:263.
- Gene ID
- 12501 View all products for this Gene ID
- UniProt
- View information about CD3e on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
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