Purified anti-human IL-16 Antibody

Pricing & Availability
Clone
14.1 (See other available formats)
Other Names
Interleukin-16
Isotype
Mouse IgG2a
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
519102 100 µg 116€
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Description

Interleukin-16 is a proinflammatory cytokine synthesized as a 68 kD precursor molecule (pro-IL-16). After cleavage of pro-IL-16 by caspase-3, IL-16 is released as a 12 kD protein, but its biologic activities are exerted by a tetramer. IL-16 is a chemoattractant for CD4+ T cells, monocytes, and eosinophils. On T cells, IL-16 upregulates CD25 and class II expression, and also inhibits HIV-1 replication in vitro by repressing the transcription of the HIV-1 long terminal repeat. IL-16 is expressed by lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts. CD4 and CD9 are the receptors of IL-16.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Peptide corresponding to amino acids 615 to 630 (RRKSLQSKETTAAGDS) of IL-16
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
ELISA, IHC-F, Block - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA2, inhibition of T cell chemotaxis2 and immunohistochemical staining of frozen lymph node sections3.

Application References
  1. Cruikshank WW, et al. 1994. P. Natl Acad Sci USA 91:5109. (ICFC)
  2. Keane J, et al. 1998. J. Immunol. 160:5945. (Block, ELISA)
  3. Chupp GL, et al. 1998. J. Immunol. 161:3114. (Block, IHC)
  4. Stoitzner P, et al. 2001. J. Invest. Dermatol. 116:641. (ICFC)
  5. Ren F, et al. 2005. J. Immunol. 174:2738. (ICFC)
Product Citations
  1. Noman M, et al. 2015. Cancer Res . 75: 3771-3787. PubMed
Publication Library
RRID
AB_11204433 (BioLegend Cat. No. 519102)

Antigen Details

Structure
12 kD protein with a PDZ domain, product of the cleavage of pro-IL-16 by caspase-3, forms tetramers.
Distribution

Lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts.

Function
Chemoattractant for CD4+ T cells, monocytes, and eosinophils. Upregulates CD25 and class II expression on T cells. Inhibits HIV-1 replication.
Ligand/Receptor
CD4.
Cell Type
Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Macrophages, Mast cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Bellon M, et al. 2010. Blood 115:2441.
2. Rofani C, et al. 2009. Stem Cells Dev. 18:671.
3. Cruikshank W and Little F. 2008. Crit. Rev. Immunol. 28:467.
4. Gu Y, et al. 2008. J. Immunol. 181:4418.
5. McFadden C, et al. 2007. J. Immunol. 179:6439.
6. Qi JC, et al. 2006. Blood 107:135.

Gene ID
3603 View all products for this Gene ID
UniProt
View information about IL-16 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04-23-2019

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