Purified anti-human CD75 Antibody

Pricing & Availability
Clone
LN1 (See other available formats)
Other Names
Beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1), B cell antigen CD75
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
LN1_Pure_102108.jpg
Human peripheral blood lymphocytes stained with purified LN1, followed by anti-mouse Igs FITC
  • LN1_Pure_102108.jpg
    Human peripheral blood lymphocytes stained with purified LN1, followed by anti-mouse Igs FITC
Cat # Size Price Quantity Avail. Save
326901 25 µg 48€
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Description

CD75 is a 43-85 kD type II transmembrane sialyltransferase protein, also known as beta-galactoside alpha-2,6-sialyltransferase. It is a member of glycosyltransferase family that transfers sialic acid from a donor substrate (CMP-sialic acid) to terminal galactose on glycan chains. CD75 is predominately expressed on germinal center originated B cells and lymphomas. It is also found on mature sIg+ B cells and epithelial cells of lung, breast, kidney, and prostate.
 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PBL nuclei
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
This antibody is at >85% purity.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P, IHC-F, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining1,2 of frozen sections and formalin-fixed paraffin-embedded sections, and Western blotting3.

Application References
  1. Epstein AL, et al.1984. J. Immunol. 133:1028. (IHC)
  2. Marder RJ, et al. 1985. Lab. Invest. 52:497. (IHC)
  3. Kramer G, et al. 2004. BJU Int. 93:822. (WB)
Product Citations
  1. Di Stefano B, et al. 2018. Nat Methods. 15:732. PubMed
Publication Library
RRID
AB_2194435 (BioLegend Cat. No. 326901)

Antigen Details

Structure
43-85 kD type II transmembrane sialoprotein, glycosyltransferase family
Distribution

Germinal center derived B cells, epithelial cells

Cell Type
B cells, Epithelial cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Epstein AL, et al. 1984. J. Immunol. 133:1028.
2. Kramer G, et al. 2004. BJU Int. 93:822.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication

Gene ID
6480 View all products for this Gene ID
UniProt
View information about CD75 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03-26-2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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