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Purified anti-human CD163 Antibody

Pricing & Availability
Clone
GHI/61 (See other available formats)
Regulatory Status
RUO
Workshop
VI M38
Other Names
GHI/61, M130, RM3/1, p155, Hemoglobin/Haptoglobin Complex Receptor, macrophage-associated antigen, ED2(rat)
Isotype
Mouse IgG1, κ
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Product Citations
publications
GHIslash61_Pure_071108.jpg
Human peripheral blood monocytes stained with purified GHI/61, followed by anti-mouse IgG FITC
  • GHIslash61_Pure_071108.jpg
    Human peripheral blood monocytes stained with purified GHI/61, followed by anti-mouse IgG FITC
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333602 100 µg 95€
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Description

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone GHI/61 binds to domain 7 of CD163. Additional reported applications (for the relevant formats) include: immunocytochemical staining, immunoprecipitation, western blot1, and spatial biology (IBEX)6,7.

Application References
  1. Pulford K, et al. 1992. Immunology 75:588. (ICC, IP, WB)
  2. Law SK, et al. 1993. Eur. J. Immunol. 23:2320.
  3. Madsen M, et al. 2004. J. Biol. Chem. 279:51561.
  4. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  5. Buttari B, et al. 2011. Atherosclerosis. 215:316. PubMed
  6. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  7. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
  2. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  3. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  4. Stras SF, et al. 2020. Developmental Cell. 51(3):357-373.e5.. PubMed
  5. Buttari B, et al. 2011. Atherosclerosis. 215:316. PubMed
  6. Camiolo MJ, et al. 2021. Cell Reports. 35(2):108974. PubMed
  7. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  8. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  9. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  10. Farhadian SF, et al. 2022. JCI Insight. 7:. PubMed
  11. Greven D, et al. 2015. Ann Rheum Dis. 74: 1924 - 1930. PubMed
  12. Kim J, et al. 2022. Sci Rep. 12:7753. PubMed
  13. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  14. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  15. Correia CR, et al. 2017. Biomater Sci. 5:551. PubMed
  16. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  17. Keskin DB, et al. 2019. Nature. 565:234. PubMed
  18. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  19. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID
AB_1088991 (BioLegend Cat. No. 333602)

Antigen Details

Structure
134 kD (non-reduced)/155 kD (reduced) glycoprotein, scavenger receptor superfamily
Distribution

Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells

Function
Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
Ligand/Receptor
Haptoglobin-hemoglobin complex, TWEAK
Cell Type
Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Roth J, et al. 1994 Transolantation. 57:127
2. Van den Heuvel MM, et al.1999 J. Leukoc. Biol. 66:858
3. Sulahian TH, et al. 2000 Cytokines 12:1312
4. Fabriek BO, et al. 2007 J. Neuroimmunol. 187:179

Gene ID
9332 View all products for this Gene ID
UniProt
View information about CD163 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 08-25-2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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