Purified anti-human CD117 (c-kit) Antibody

Pricing & Availability
Clone
104D2 (See other available formats)
Other Names
Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
104D2_Purified_071007
Human myeloid cell line TF-1 stained with purified 104D2, followed by biotinylated anti-mouse IgGs and Sav-PE
  • 104D2_Purified_071007
    Human myeloid cell line TF-1 stained with purified 104D2, followed by biotinylated anti-mouse IgGs and Sav-PE
Cat # Size Price Quantity Avail. Save
313201 25 µg 52€
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313202 100 µg 120€
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Description

CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Cynomolgus, Cattle (Bovine, Cow)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
MOLM-1 megakaryocytic cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
ICC, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and immunofluorescence microscopy1.

Application References
  1. Broudy VC, et al. 1999. Blood 94:1979. (IF, IP)
  2. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  3. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
Product Citations
  1. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  2. Vigneault P, et al. 2018. J Physiol. 596:2359. PubMed
  3. Jokela TA, et al. 2018. Front Cell Dev Biol. 6:41. PubMed
  4. Ortiz AM, et al. 2018. Nat Med. 24:1313. PubMed
  5. Ardain A, et al. 2019. Nature. 570:528. PubMed
  6. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
  7. Lowinus T, et al. 2019. Cell Commun Signal. 17:05. PubMed
  8. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  9. Nagano M, et al. 2007. Blood . 110:151. PubMed
  10. Guo A, et al. 2011. J Pharmacol Exp Ther. 338:421. PubMed
  11. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. PubMed
RRID
AB_314980 (BioLegend Cat. No. 313201)
AB_314981 (BioLegend Cat. No. 313202)

Antigen Details

Structure
Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
Distribution

Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)

Function
Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
Ligand/Receptor
c-Kit ligand
Modification
Multiple phosphorylation sites
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.

Gene ID
3815 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04-30-2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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