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Purified anti-Granzyme B Antibody

Pricing & Availability
Clone
M3304B06 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase, CSPB, CTLA-1, CGL-1, CGL1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a-M3304B06_Purified_Granzyme_B_Antibody_WB_122414
Western blot analysis of PBMC (lane 1), PBMC activated with 5 µg/ml CD3 antibody coated on the plate and 2 µg/ml CD28 antibody for two days (lane 2) using anti-Granzyme B antibody (clone M3304B06). Anti-β actin antibody (poly6221) was used as a loading control.
  • a-M3304B06_Purified_Granzyme_B_Antibody_WB_122414
    Western blot analysis of PBMC (lane 1), PBMC activated with 5 µg/ml CD3 antibody coated on the plate and 2 µg/ml CD28 antibody for two days (lane 2) using anti-Granzyme B antibody (clone M3304B06). Anti-β actin antibody (poly6221) was used as a loading control.
  • b-M3304B06_Purified_Granzyme_B_Antibody_IHC_121322
    IHC staining of purified anti-Granzyme B antibody (clone M3304B06) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Tris-EDTA buffer (10mM Tris, 1mM EDTA, pH 9.0), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4&Deg;C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (magenta) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slides were mounted with ProLong™ Gold Antifade Mountant. Scale bar: 50 µm. B: Crop image of A. Scale bar: 10 µm
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674602 100 µg 240€
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Description

Granzyme B is a serine protease that is mainly produced by cytotoxic T cells (CTL) and NK cells. It plays important roles in cytotoxic lymphocyte-mediated apoptosis, chronic inflammation, and impaired wound healing. Granzyme B is packaged in cytoplasmic granules that are exocytosed towards a bound target cell, and subsequently activates multiple protein substrates to induce apoptosis. Perforin facilitates the transfer of Granzyme B into the target cell. Most circulating CD56+ CD8- NK cells and approximately half of circulating CD8+ T cells coexpress both Granzyme A and B. The activation of CD8+ and CD4+ T lymphocytes induces substantial expression of Granzyme B, but not Granzyme A. Recently, Granzyme B has been found to be produced by other cell types such as CD34+ hematopoietic progenitor cells, keratinocytes, basophils, mast cells, plasmacytoid dendritic cells, B cells, and smooth muscle cells. Elevated levels of Granzyme B are also found in some kinds of autoimmune diseases, type 1 diabetes, and cardiovascular diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Granzyme B produced in the 293E cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Spolski R et al. 2019. Elife. 8 pii: e45501. PubMed
RRID
AB_2565266 (BioLegend Cat. No. 674602)

Antigen Details

Structure
247 amino acids with a molecular weight of approximately 28 kD
Distribution

Cytotoxic T cells, NK cells, and neutrophils.

Function
Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA / IL-2 and CD4+ T lymphocytes with anti-CD3/CD28 or anti-CD3/CD46.
Interaction
Targets of CTL and NK cells.
Cell Type
B cells, Dendritic cells, Neutrophils, NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Molecular Family
Enzymes and Regulators, Proteases
Antigen References

1. Edwards KM, et al. 1999. J. Biol. Chem. 274:30468.
2. Grossman WJ, et al. 2004. Blood 104:2840.
3. Heusel JW, et al. 1994. Cell 76:977.
4. Schmid J and Weissmann C. 1987. J. Immunol. 139:250.
5. Trapani JA, et al. 1988. Proc. Natl. Acad. Sci. USA 85:6924.
6. Hiebert PR and Granville DJ. 2012. Trends Mol. Med. 18:732.
7. Saito Y, et al. 2011. J. Cardiol. 57:141.
8. Ewen CL, et al. 2012. Cell Death Differ. 1:28.

Gene ID
3002 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 12-13-2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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