- 9E11 (See other available formats)
- Regulatory Status
- Other Names
- Myc proto-oncogene protein, proto-oncogene c-Myc, transcription factor p64, class E basic helix-loop-helix protein 39, avian myelocytomatosis viral oncogene homolog, v-myc, myc-related translation/localization
Covance Catalog# MMS-164P
- Mouse IgG2a
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- Product Citations
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c-Myc is a naturally occurring protein that is often used as a protein modification and tag in order to simplify the labeling and detection of proteins. Antibodies against c-Myc recognize both naturally occurring c-Myc and overexpressed proteins containing the c-Myc epitope tag fused to targeted proteins allowing for specific detection in western blotting, immunoprecipitation, and immunostaining techniques.Product Details
- Antibody Type
- Host Species
- Phosphate-buffered solution + 0.03% Thimerosal.
- The antibody was purified by affinity chromatography.
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
WB - Quality tested
ICC, IHC-P, IP - Verified
Purification - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The 9E11 monoclonal antibody recognizes the sequence 408-420 AEEQKLISEEDL of human c-myc.
This clone recognizes a non-specific band in BL21 E. coli lysates
BioLegend offers two clones against c-Myc, 9E10 and 9E11. When both clones were tested in parallel for WB, ICC, and IP, each clone performed similarly, with 9E11 showing slightly better performance for each application.
- Application References
- Halme A, et al. 2004. Cell. 116:405.
- Kolodziej P, Young R. 1991. Meth. Enzymol. 194:508.
- Locker AP, et al. 1989. Brit. J. Cancer. 60:669.
- Product Citations
AB_2565039 (BioLegend Cat. No. 904401)
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