Purified anti-β-Amyloid, x-42 Antibody (Previously Covance catalog# SIG-39169)

Pricing & Availability
Clone
1-11-3 (See other available formats)
Other Names
Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Covance Catalog# SIG-39169
Isotype
Rabbit IgG
Ave. Rating
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Product Citations
publications
1-11-3_PURE_B-Amyloid_Antibody_IHC_062818
IHC staining of purified anti-β-Amyloid, x-42 antibody (clone 1-11-3) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1-11-3_PURE_B-Amyloid_Antibody_IHC_062818
    IHC staining of purified anti-β-Amyloid, x-42 antibody (clone 1-11-3) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
812101 50 µg 294€
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Description

Alzheimer’s disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor protein (APP) through sequential proteolytic cleavage of APP by β- and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunohistochemistry (IHC-P) and immunoblotting (WB).


This antibody is reactive to the C-terminus of beta amyloid and specific for the isoform that ends at the 42nd amino acid.

Application References
  1. Currais A, et al. 2012. Aging Cell 6:1017. (IHC) PubMed
  2. Mastrangelo MA, Bowers WJ. 2008. BMC Neurosci. 9:81. (IHC) PubMed
Product Citations
  1. Bowers M 2008. BMC Neurosci. 9:81. PubMed
  2. Currais A, et al. 2012. Aging Cell. 11:1017-1026. PubMed
Publication Library
RRID
AB_2564763 (BioLegend Cat. No. 812101)
Disclaimer

Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics.

Antigen Details

Structure
Expected MW: 4 kD
Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid, x-42 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06-28-2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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