Purified anti-β-actin Antibody

Pricing & Availability
Clone
2F1-1 (See other available formats)
Regulatory Status
RUO
Other Names
Actin, cytoplasmic 1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A_2F1-1_Purified_Beta-Actin_Antibody_1_WB_080316
Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 cells (lane 2, 15 µg), U87-MG (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-β actin antibody (clone 2F1-1). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
  • A_2F1-1_Purified_Beta-Actin_Antibody_1_WB_080316
    Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 cells (lane 2, 15 µg), U87-MG (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-β actin antibody (clone 2F1-1). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
  • B_2F1-1_PURE_BetaActin_Ab_IF_022018
    Day-three cultured postnatal C57BL/6 mouse brain cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/ml of purified anti- β-actin (clone 2F1-1) in blocking buffer, overnight at 4°C, followed by Alexa Fluor® 594 anti-mouse IgG (red) staining for 2 hours at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
Cat # Size Price Quantity Avail. Save
643801 25 µg 68,00€
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643802 100 µg 156,00€
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Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µL per 5 mL antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol.

Application References
  1. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  2. Joyce CW, et al. 2006. J. Biol. Chem. 281:33053.
  3. Yanagiya T,et al.2007.Obesity.15:572.
  4. KishidaT,et al. 2007.J. Immunol. 179:8554.
  5. Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144.
  6. Toltlt LJ,et al. 2008.J. Immunol. 181:2165.
  7. Sawada. T,et al. 2008. J. Biol. Chem. 283:26820.
  8. Ikeda D, et al. 2008. Endocrinology. 149:6037.
  9. Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed
  10. Kaieda S, et al. 2010. J. Biol Chem. 285:21478. PubMed
  11. Cambos M, et al. 2011. J. Leukoc. Biol. 89:157. PubMed
Product Citations
  1. Qin S, et al. 2018. Oncol Lett. 128:1283. PubMed
  2. Hadjicharalambous MR, et al. 2019. Sci Rep. 9:6020. PubMed
  3. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  4. Budida R, et al. 2017. Eur J Immunol. 47:1819. PubMed
  5. Lalor R, et al. 2019. Nutrients. 11. PubMed
  6. Lovatt M, et al. 2018. Antioxidants (Basel). 0.416666667. PubMed
  7. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
  8. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  9. Kaieda S, et al. 2010. J Biol Chem. 285:21478. PubMed
  10. Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed
  11. Scorza M 2011. J Leukoc Biol. 89:159. PubMed
  12. Greenberg D, et al. 2015. Infect Immun . 83: 4277 - 4292. PubMed
  13. Bruggen R, et al. 2015. PLoS One. 10: 0144555. PubMed
  14. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
RRID
AB_2223201 (BioLegend Cat. No. 643801)
AB_2223199 (BioLegend Cat. No. 643802)

Antigen Details

Structure
Actin, cytoplasmic 1
Distribution

Ubiquitously expressed in the cytoplasm of all eukaryotic cells

Function
Actins are highly conserved proteins that are involved in cell motility
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Antigen References

1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.

Gene ID
11461 View all products for this Gene ID
UniProt
View information about beta-actin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 11-05-2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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