- LCK-01 ; A18002D ;
- Regulatory Status
- Other Names
- LSK; 56lck; LCK Proto-Oncogene, Src Family Tyrosine Kinase; p56LCK; Pp58Lck T Cell-Specific Protein Tyrosine Kinase
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- Product Citations
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The Src family tyrosine kinase p56Lck (Lck) is a non-receptor tyrosine kinase that plays a critical role in T cell selection and maturation within the thymus, and also in the function of mature T cells. Lck, which is constitutively bound to cytosolic domains of CD4 and CD8 surface receptors, plays an essential role in T cell receptor (TCR) signaling. Engagement of the TCR with peptide antigen-loaded MHC complexes results in the recruitment of CD4- and CD8-bound Lck to the TCR/CD3 signaling complex. Lck then transphosphorylates TCR-gamma chains and CD3 subunits, thereby activating the TCR/CD3 signaling pathway and leading to the recruitment and subsequent phosphorylation of Zap70 by Lck. Lck also plays an important role in interleukin-2 signaling that regulates the T cell proliferative response. Phosphorylation of Lck by CSK at tyrosine 505 negatively regulates the kinase, and is proposed to generate a closed, inactive conformation of the protein. Conversely, phosphorylation at tyrosine 394 in the activation loop of Lck greatly stimulates enzymatic activity. In many types of cancer, Lck is a proliferative and anti-apoptotic driver, and has been proposed as a target for therapeutic intervention.Product Details
- Kit Contents
Product Application Clone Size Reactivity Isotype Purified anti-Lck Antibody WB, ICC, IP LCK-01 25 μg Human Mouse IgG1, κ Purified Lck Phospho (Tyr394) Antibody WB, ICC, ICFC A18002D 25 μg Human, Mouse Mouse IgG1, κ
* For detailed information about each specificity, please refer to the datasheets of the individual products.
- Please refer to individual product datasheets for immunogen information
- Phosphate-buffered solutions, pH 7.2, containing 0.09% sodium azide
- The antibodies were purified by affinity chromatography.
- Storage & Handling
- The antibody solutions should be stored undiluted between 2°C and 8°C.
WB - Quality tested
- Recommended Usage
Each lot of antibodies in this set is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:
Clone LCK-01: 1.0 µg/mL
Clone A18002D: 0.1 - 1.0 µg/mL
It is recommended that the reagent be titrated for optimal performance for each application.
For recommended usage information for additional applications, please refer to the datasheet of the individual products.
- Lck is a 509 amino acid protein with a predicted molecular weight of ~58 kD.
T cells/Plasma membrane-associated
- T cell receptor signaling
- Antigen References
- Phillipsen L, et al. 2017. Sci Signal. 10:eaaf4736.
- Moogk D, et al. 2016. J Immunol. 197:644.
- Klammt C, et al. 2015. Nat Immunol. 16:961.
- Cancer Genome Atlas Network. 2015. Cell. 161:1681.
- Casas J, et al. 2014. Nat Commun. 5:5624.
- McNeill L, et al. 2007. Immunnity. 27:425.
- Lefebvre DC, et al. 2003. Biochim Biophys Acta. 1650:40.
- Kabouridis PS. et al. 2003. Biochem J. 371:907.
- Abraham N and Veillette 1990. Mol. Cell. Biol. 10:5197.
- Gene ID
- 3932 View all products for this Gene ID
- View information about Lck on UniProt.org
|PhosphoPair Lck (Tyr394) Antibody Set||LCK-01; A18002D||WB|
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