PerCP/Cyanine5.5 anti-mouse Siglec H Antibody

Pricing & Availability
Clone
551 (See other available formats)
Regulatory Status
RUO
Other Names
Sialic acid-binding immunoglobulin-like lectin
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
551_PerCPCy5.5_1_090310
C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) Alexa Fluor® 647and 551 PerCP/Cyanine5.5
  • 551_PerCPCy5.5_1_090310
    C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) Alexa Fluor® 647and 551 PerCP/Cyanine5.5
  • 551_PerCPCy5.5_2_090310
    C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) Alexa Fluor® 647 and rat IgG1 PerCP/Cyanine5.5 isotype control
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
129613 25 µg 84,00€
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129614 100 µg 228,00€
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Description

The Siglec-H is a member of CD33-related Siglec family which mostly expressed in the innate immune system. Siglec-H is reported to be specifically expressed on plasmacytoid dendritic cells. Siglec-H depends on adaptor protein DAP 12 (DNAX-activating protein of 12 kD) for surface expression and signal transduction. Cross-linking with this antibody can selectively inhibit interferon-alpha production by IPCs following TLR9 ligation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Siglec-H-Fc recombinant fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated withPerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. Blasius AL, et al. 2006. J. Immunol. 177:3260.
Product Citations
  1. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  2. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  3. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
RRID
AB_10639936 (BioLegend Cat. No. 129613)
AB_10643995 (BioLegend Cat. No. 129614)

Antigen Details

Structure
Sialic acid-binding immunoglobulin-like lectins
Distribution

Siglec-H is expressed exclusively on natural interferon-producing cells (IPCs), also called plasmacytoid dendritic cells in spleen, blood and lymph nodes. The IPCs are characterized by a complex phenotype (CD11c+, Ly-6C+, B220+, CD11b-, CD8+/-).

Function
Siglec-H associates with adaptor protein DAP12 for cell signaling, which reduces type 1 IFN response of IPC to CpG.
Ligand/Receptor
Sialic acid
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Blasius AL, et al. 2006. Blood 107:2474.
2. Zhang J, et al. 2006. Blood 107:3600.
3. Blasius AL, et al. 2007. J. Exp. Med. 204:2561.

Gene ID
233274 View all products for this Gene ID
UniProt
View information about Siglec H on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11-30-2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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