- 4B12 (See other available formats)
- Regulatory Status
- Other Names
- C-C chemokine receptor type 7 (CCR7), MIP-3 beta receptor, EBV-induced G protein coupled receptor 1, EBI-1, CD197
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD197 is also known as C-C chemokine receptor 7 (CCR7) or EBI-1. CCR7 is a G-coupled rhodopsin-like member of the GPCR superfamily with a predicted molecular weight of 43 kD that is expressed on hematopoietic stem cells, most naive T cells, some memory T cells, B subset, and mature dendritic cells. CCR7 is a receptor for the chemokines CCL19 (MIP3 beta) and SLC (6CKine, Exodus-2, TCA-4, CCL21) that plays a role in thymocytes development, T cell adhesion at intestinal sites, the homeostatic recirculation of memory T cells, and chemotaxis.Product Details
- Antibody Type
- Host Species
- Mouse CCR7 transfected RBL-2H3 cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 2.0 µg per 106cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.
Staining with clone 4B12 is recommended at 37°C (see supplemental data of PE staining at differing temperatures).
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
- Ohl L, et al. 2004. Immunity 21:279.
- Ritter U, et al. 2004. J. Leukocyte Biol. 76:472.
- Lan YY, et al. 2005. Am. J. Transplant. 5:2649. (FC)
- Lee JH, et al. 2007. J. Immunol. 178:301. (FC) PubMed
- Kurooka M and Kaneda Y. 2007. Cancer Res. 67:227. (FC) PubMed
- Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC)
- Sakai N, et al. 2006. P. Natl. Acad. Sci. USA 103:14098. (FC)
- Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC)
- Hwang IY, et al. 2007. J. Immunol. 179:439. (FC) PubMed
- Kang SG, et al. 2007. J. Immunol. 179:3724.
- Mao A et al. 2005. J. Immunol. 175:5146. PubMed
- Allende ML, et al. 2008. FASEB J. 22:307. PubMed
- Kang SG, et al. 2007. J. Immunol. 179:3724. PubMed
- Chen H, et al. 2005. J. Immunol. 175:591. PubMed
- Florido M, et al. 2009. Immunobiology. 214:643. PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
- Product Citations
AB_2228801 (BioLegend Cat. No. 120115)
AB_2291144 (BioLegend Cat. No. 120116)
- Rhodopsin-like GPCR superfamily, G-protein coupled receptor 1 family, integral membrane protein, predicted molecular weight 43 kD
Hematopoietic stem cells, T subsets, mature dendritic cells
- Homozygous mutation. Receptor for MIP-3 beta and SLC chemokines. Probable mediator of EBV effects on B lymphocytes. Plays a role in T cell adhesion at intestinal sites; may also play a role in the homeostatic recirculation of memory T cells and chemotaxis.
- MIP3 beta, SLC (6CKine, Exodus-2, TCA-4)
- Cell Type
- Hematopoietic stem and progenitors, T cells, Dendritic cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
1. Schweickart VL, et al. 1994. Genomics 23:643.
2. Yoshida R, et al. 1998. J. Biol. Chem. 273:7118.
3. Campbell JJ, et al. 1998. J. Cell Biol. 141:1053.
4. Willimann K, et al. 1998. Eur. J. Immunol. 28:2025.
- Gene ID
- 12775 View all products for this Gene ID
- View information about CD197 on UniProt.org
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|Purified anti-mouse CD197 (CCR7)||4B12||FC, IP|
|Biotin anti-mouse CD197 (CCR7)||4B12||FC|
|PE anti-mouse CD197 (CCR7)||4B12||FC|
|APC anti-mouse CD197 (CCR7)||4B12||FC|
|Alexa Fluor® 647 anti-mouse CD197 (CCR7)||4B12||FC|
|Brilliant Violet 785™ anti-mouse CD197 (CCR7)||4B12||FC|
|Brilliant Violet 421™ anti-mouse CD197 (CCR7)||4B12||FC|
|PE/Dazzle™ 594 anti-mouse CD197 (CCR7)||4B12||FC|
|PE/Cyanine7 anti-mouse CD197 (CCR7)||4B12||FC|
|Brilliant Violet 605™ anti-mouse CD197 (CCR7)||4B12||FC|
|Alexa Fluor® 488 anti-mouse CD197 (CCR7)||4B12||FC|
|PE/Cyanine5 anti-mouse CD197 (CCR7)||4B12||FC|
|TotalSeq™-C0377 anti-mouse CD197 (CCR7)||4B12||PG|
|TotalSeq™-B0377 anti-mouse CD197 (CCR7)||4B12||PG|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD197 (CCR7)
Biotin anti-mouse CD197 (CCR7)
PE anti-mouse CD197 (CCR7)
APC anti-mouse CD197 (CCR7)
Alexa Fluor® 647 anti-mouse CD197 (CCR7)
Brilliant Violet 785™ anti-mouse CD197 (CCR7)
Brilliant Violet 421™ anti-mouse CD197 (CCR7)
PE/Dazzle™ 594 anti-mouse CD197 (CCR7)
PE/Cyanine7 anti-mouse CD197 (CCR7)
Brilliant Violet 605™ anti-mouse CD197 (CCR7)
Alexa Fluor® 488 anti-mouse CD197 (CCR7)
PE/Cyanine5 anti-mouse CD197 (CCR7)
TotalSeq™-C0377 anti-mouse CD197 (CCR7)
TotalSeq™-B0377 anti-mouse CD197 (CCR7)