PerCP/Cyanine5.5 anti-human CD294 (CRTH2) Antibody

Pricing & Availability
Clone
BM16 (See other available formats)
Regulatory Status
RUO
Other Names
Chemoattractant receptor-homologous molecule expressed on Th2 cells, GPR44, G-protein coupled receptor 44, DP2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
BM16_PerCPCyanine55_CD294_Antibody_082218
Human peripheral blood lymphocytes were stained with CD4 FITC, CD194 (CCR4) APC and CD294 (CRTH2) (clone BM16) PerCP/Cyanine5.5 (left), or rat IgG2a, ? PerCP/Cyanine5.5 isotype control (right). Data shown was gated on the CD4+ population.
  • BM16_PerCPCyanine55_CD294_Antibody_082218
    Human peripheral blood lymphocytes were stained with CD4 FITC, CD194 (CCR4) APC and CD294 (CRTH2) (clone BM16) PerCP/Cyanine5.5 (left), or rat IgG2a, ? PerCP/Cyanine5.5 isotype control (right). Data shown was gated on the CD4+ population.
Compare all formats See PerCP/Cyanine5.5 spectral data
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350115 25 tests 96,00€
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350116 100 tests 236,00€
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Description

CD294, also known as CRTH2, is a seven-transmembrane protein coupled with heterotrimeric G proteins. CRTH2 is the prostaglandin D2 receptor and is expressed by Th2 cells, eosinophils, and basophils. CD294 prevents the apoptosis of Th2 cells and mediates the chemotaxis of CRTH2 expressing cells to the sites of allergic inflammation, such as the asthmatic lung.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Rat cell line TART/B19-12.10 transfected with human CRTH2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

This product is sold under license agreement with BML, Inc for research use only.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. Nagata K, et al. 1999. J. Immunol. 162:1278. (FC)
  2. De Fanis U, et al. 2007. Blood 109:4343. (FC)
  3. Tsuda H, et al. 2001. Clin. Exp. Immunol. 123:105. (FC)
  4. Hamada K, et al. 2004. Allergology International 53:179. (FC)
RRID
AB_2562466 (BioLegend Cat. No. 350115)
AB_2562467 (BioLegend Cat. No. 350116)

Antigen Details

Structure
Seven-transmembrane protein coupled with G proteins
Distribution

Effector T helper 2 (Th2) cells, eosinophils, basophils

Function
Chemotaxis of eosinophils, basophils and Th2 cells, prevention of apoptosis in Th2 cells
Interaction
Heterotrimeric G proteins
Ligand/Receptor
Prostaglandin D2
Cell Type
Th2, Eosinophils, Basophils
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
CD Molecules
Antigen References

1. Luster AD and Tager AM. 2004. Nat. Rev. Immunol. 4:711.
2. Monneret G, et al. 2001. Blood 98:1942.
3. Xue L, et al. 2009. J. Immunol. 182:7580.
4. Schratl P, et al. 2007. J. Immunol. 179:4792.

Gene ID
11251 View all products for this Gene ID
UniProt
View information about CD294 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 08-22-2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD294 (CRTH2)

  • Alexa Fluor® 647 anti-human CD294 (CRTH2)

  • PE anti-human CD294 (CRTH2)

  • FITC anti-human CD294 (CRTH2)

  • APC anti-human CD294 (CRTH2)

  • Brilliant Violet 421™ anti-human CD294 (CRTH2)

  • APC/Cyanine7 anti-human CD294 (CRTH2)

  • PerCP/Cyanine5.5 anti-human CD294 (CRTH2)

  • PE/Cyanine7 anti-human CD294 (CRTH2)

  • Brilliant Violet 510™ anti-human CD294 (CRTH2)

  • Brilliant Violet 605™ anti-human CD294 (CRTH2)

  • Brilliant Violet 711™ anti-human CD294 (CRTH2)

  • PE/Dazzle™ 594 anti-human CD294 (CRTH2)

  • TotalSeq™-A0102 anti-human CD294 (CRTH2)

  • Pacific Blue™ anti-human CD294 (CRTH2)

  • TotalSeq™-C0102 anti-human CD294 (CRTH2)

  • APC/Fire™ 750 anti-human CD294 (CRTH2) Antibody

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