PerCP/Cyanine5.5 anti-human CD181 (CXCR1) Antibody

Pricing & Availability
Clone
8F1/CXCR1 (See other available formats)
Regulatory Status
RUO
Other Names
CD181, CXCR1, CDw128, CD128a, IL-8RA, Type I IL-8 receptor
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
8F1CXCR1_PerCPCyanine55_CD181_Antibody_012919
Human peripheral blood granulocytes were stained with CD181 (CXCR1)(clone 8F1/CXCR1) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2b, ? PerCP/Cyanine5.5 isotype control (open histogram).
  • 8F1CXCR1_PerCPCyanine55_CD181_Antibody_012919
    Human peripheral blood granulocytes were stained with CD181 (CXCR1)(clone 8F1/CXCR1) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2b, ? PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
320622 100 tests 260€
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Description

CXCR1 is a 67-70 kD seven-transmembrane protein, also known as IL-8 receptor A (IL-8RA), CD181, and CDw128a. It is a CXC chemokine receptor that belongs to the G protein-coupled receptor (CPCR) family. CXCR1 is expressed as homodimer or heterodimer with CXCR2 and found on granulocytes, NK cells, subset of T lymphocytes, mast cells, monocytes, endothelial cells, megakarocytes, and oligodendrocytes. The expression level of CXCR1 on monocytes is lower than that of CXCR2. CXCR1 mediates neutrophil activation and chemotaxis, megakaryocytic proliferation, and angiogenesis via binding its ligands including IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), and GRO-α(CXCL1).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CXCR1 transfected L1.2 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: The 8F1/CXCR1 antibody is useful for immunofluorescent staining and flow cytometric analysis of CXCR1 expression.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
RRID
AB_2565552 (BioLegend Cat. No. 320622)

Antigen Details

Structure
Seven transmembrane spanning, G protein-coupled receptor (CPCR), 67-70 kD, homodier or teterodimer with CXCR2
Distribution

Granulocytes, NK cells, subset of T cells, monocytes, endothelial cells, oligodendrocytes

Function
neutrophil activation, chemotaxis, angiogenesis
Ligand/Receptor
IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), GRO-a(CXCL1)
Cell Type
Granulocytes, NK cells, T cells, Monocytes, Endothelial cells, Oligodendrocytes, Neutrophils
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Chuntharapai A, et al. 1994. J. Immunol. 153:5682.
2. Wilson S, et al. 2005. J. Biol. Chem. 280:28663.
3. Emadi S, et al. 2005. Blood 105:464.
4. Omari KM, et al. 2005. Brain 128:1003.
5. Juremalm M, G. Nilsson. 2005. Chem. Immunol. Allergy. 87:130.
6. Wolf M, et al. 1998. Eur. J. Immunol. 28:164

Gene ID
3577 View all products for this Gene ID
UniProt
View information about CD181 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 03-17-2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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