PE/Cy7 anti-human/mouse SSEA-3 Antibody

Pricing & Availability
Clone
MC-631 (See other available formats)
Other Names
Stage-Specific Embryonic Antigen-3
Isotype
Rat IgM, κ
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Product Citations
publications
MC-631_PECy7_SSEA-3_Antibody_022118
NCCIT (human teratocarcinoma cell line) were stained with anti-human/mouse SSEA-3 (clone MC-631) PE/Cy7 (filled histogram) or rat IgG1, ĸ PE/Cy7 (open histogram).
  • MC-631_PECy7_SSEA-3_Antibody_022118
    NCCIT (human teratocarcinoma cell line) were stained with anti-human/mouse SSEA-3 (clone MC-631) PE/Cy7 (filled histogram) or rat IgG1, ĸ PE/Cy7 (open histogram).
See PE/Cy7 spectral data
Cat # Size Price Quantity Avail. Save
330325 25 tests 108€
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330326 100 tests 272€
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Description

Stage-Specific Embryonic Antigen-3 (SSEA-3) is an internal core structure of glob-series glycolipids (R-Galβ1-3GalNAcβ1-3Galα1-4Galβ1-R). SSEA-3 is expressed on human teratocarcinoma cells (embryonal carcinoma, EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-3 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
4-8 cell stage mouse embryos
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Kannagi R, et al. 1983. EMBO 2:2355. (IF)
  2. Kaufmann B, et al. 2005. Virology 332:189. (ELISA)
RRID
AB_2728283 (BioLegend Cat. No. 330325)
AB_2728284 (BioLegend Cat. No. 330326)

Antigen Details

Distribution

Embryonic stemcells

Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Stem Cells
Gene ID
93961 View all products for this Gene ID
UniProt
View information about SSEA-3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02-26-2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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