PE Rat IgG2b, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK4530 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
400607 25 µg 28,00€
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400635 25 tests 36,00€
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400608 100 µg 60,00€
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400636 100 tests 64,00€
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Description

The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
test sizes: lot-specific; µg sizes: 0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this rat IgG2b, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin <  0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.

Application References
  1. Cervantes-Barragan L, et al. 2007. Blood 109:1131.
  2. Zeiser R, et al. 2007. Blood 109:2225.
  3. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed
  4. Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed
  5. Yi H, et al. 2009. Blood 113:5819. PubMed
  6. Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed
  7. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
  8. Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
Product Citations
  1. Hao J et al. 2018. Cell metabolism. 28(5):689-705 . PubMed
  2. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  3. Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed
  4. Xu X, et al. 2018. J Dermatol Sci. 91:134. PubMed
  5. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  6. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  7. Miao L, et al. 2020. Theranostics. 0.7625. PubMed
  8. Mul Fedele ML, et al. 2020. Front Cell Infect Microbiol. 10:100. PubMed
  9. Zhang M, et al. 2019. Autophagy. 1.423611111. PubMed
  10. Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed
  11. Fukushima K, et al. 2020. Immunity. 52(3):542-556. PubMed
  12. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
  13. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  14. Krishnamurthy B, et al. 2012. Diabetes. 61:425. PubMed
  15. Graham K, et al. 2011. Am J Pathol. 178:2716. PubMed
  16. Madera L, et al. 2015. PLoS One. 10: 0133385. PubMed
  17. Chen Y, et al. 2016. PLoS One. 11: 0149233. PubMed
  18. Ojeda-Fernández L, et al. 2016. PLoS One. 12: 1005935. PubMed
  19. Iwasaki Y, et al. 2016. PLoS One. 11: 0158282. PubMed
  20. Fan C, et al. 2020. Stem Cell Res Ther. 0.765277778. PubMed
  21. Yang M, et al. 2020. Appl Environ Microbiol. 86:00:00. PubMed
  22. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  23. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  24. Byun JK, et al. 2020. Molecular Cell. 80(4):592-606.e8. PubMed
  25. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  26. Garg M, et al. 2021. Cell Reports. 34(6):108736. PubMed
  27. Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed
  28. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
RRID
AB_326551 (BioLegend Cat. No. 400607)
AB_326552 (BioLegend Cat. No. 400608)
AB_893669 (BioLegend Cat. No. 400636)

Antigen Details

Structure
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Gene ID
NA

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All Reagents Request Custom Conjugation
Description Clone Applications
APC Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
Biotin Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
FITC Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
PE Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
PE/Cyanine5 Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
Purified Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC, ICC, IHC, IP, WB
PE/Cyanine7 Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
APC/Cyanine7 Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
Alexa Fluor® 647 Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
Alexa Fluor® 488 Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
Pacific Blue™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
Alexa Fluor® 700 Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
PerCP Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
Brilliant Violet 421™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
Brilliant Violet 570™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC, ICC, IHC, IP, WB, FA
Brilliant Violet 510™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
Brilliant Violet 605™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
Brilliant Violet 785™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
Brilliant Violet 650™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
Brilliant Violet 711™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
PE/Dazzle™ 594 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Alexa Fluor® 594 Rat IgG2b, κ Isotype Ctrl RTK4530 IHC-F
GoInVivo™ Purified Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC, ICC, IHC, IP, WB, FA
APC/Fire™ 750 Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
TotalSeq™-A0095 Rat IgG2b, κ Isotype Ctrl RTK4530 PG
TotalSeq™-C0095 Rat IgG2b, κ Isotype Ctrl RTK4530 PG
KIRAVIA Blue 520™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark Blue™ 550 Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
PE/Fire™ 640 Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
APC/Fire™ 810 Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
PE/Fire™ 700 Rat IgG2b, κ Isotype Ctrl RTK4530 FC, ICFC
TotalSeq™-B0095 Rat IgG2b, κ Isotype Ctrl RTK4530 PG
Go To Top Version: 1    Revision Date: 11-30-2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC Rat IgG2b, κ Isotype Ctrl

  • Biotin Rat IgG2b, κ Isotype Ctrl

  • FITC Rat IgG2b, κ Isotype Ctrl

  • PE Rat IgG2b, κ Isotype Ctrl

  • PE/Cyanine5 Rat IgG2b, κ Isotype Ctrl

  • Purified Rat IgG2b, κ Isotype Ctrl

  • PE/Cyanine7 Rat IgG2b, κ Isotype Ctrl

  • APC/Cyanine7 Rat IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 647 Rat IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 488 Rat IgG2b, κ Isotype Ctrl

  • Pacific Blue™ Rat IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 700 Rat IgG2b, κ Isotype Ctrl

  • PerCP Rat IgG2b, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 421™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 570™ Rat IgG2b, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 510™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 605™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 785™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 650™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 711™ Rat IgG2b, κ Isotype Ctrl

  • PE/Dazzle™ 594 Rat IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 594 Rat IgG2b, κ Isotype Ctrl

  • GoInVivo™ Purified Rat IgG2b, κ Isotype Ctrl

  • APC/Fire™ 750 Rat IgG2b, κ Isotype Ctrl

  • TotalSeq™-A0095 Rat IgG2b, κ Isotype Ctrl

  • TotalSeq™-C0095 Rat IgG2b, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Rat IgG2b, κ Isotype Ctrl

  • Spark Blue™ 550 Rat IgG2b, κ Isotype Ctrl

  • PE/Fire™ 640 Rat IgG2b, κ Isotype Ctrl

  • APC/Fire™ 810 Rat IgG2b, κ Isotype Ctrl

  • PE/Fire™ 700 Rat IgG2b, κ Isotype Ctrl

  • TotalSeq™-B0095 Rat IgG2b, κ Isotype Ctrl

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