- 5E8 (See other available formats)
- Regulatory Status
- Other Names
- CC CKR3, MIP1-alpha receptor like-2, eotaxin receptor, CD193, CCR3
- Mouse IgG2b, κ
- Ave. Rating
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- Product Citations
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CD193, also known as CC-chemokine receptor 3 (CCR3), CC CKR3, MIP1-alpha receptor like-2, and eotaxin receptor, is a member of the G protein-coupled seven transmembrane receptors family. It binds to the CC chemokines eotaxin, eotaxin-2, and eotaxin-3 with high affinity. CCR3 has also been reported to bind RANTES, MCP-3, and MCP-4 with low affinity. CCR3 receptor is expressed on human eosinophils, basophils, mast cells, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitor cells, Th2-like lymphocytes, and keratinocytes. CCR3 is thought to play a role in allergic diseases such as bronchial asthma and allergic rhinitis. CCR3 is a co-receptor for HIV-1 and HIV-2, and the binding of eotaxin with CCR3 has been shown to inhibit HIV infection in some cell types.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: The 5E8 antibody is useful for immunofluorescent staining and flow cytometric analysis of CCR3 expression.
It has been observed that the 5E8 antibody clone can interact with PE/Cy7 antibody conjugates during multi-color staining, potentially leading to unwanted staining. This interaction can be resolved by sequentially staining with the 5E8 antibody first and then followed by the PE/Cy7 conjugate of interest.
- Application References
- Beauvillian C, et al. 2011. Blood 117:1196. PubMed
AB_2687006 (BioLegend Cat. No. 310727)
AB_2687007 (BioLegend Cat. No. 310728)
- G-protein coupled seven transmembrane domain receptor, 356 amino acids
Eosinophils, basophils, mast, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitor, Th2, keratinocytes
- Co-receptor for HIV-1 and HIV-2, allergy
- Eotaxin, eotaxin-2, eotaxin-3
- Cell Type
- Eosinophils, Erythrocytes, Hematopoietic stem and progenitors, Macrophages, Mast cells, Thymocytes
- Biology Area
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
1. Gerard W, et al. 1996. J. Exp. Med. 183:2437.
2. Uguccioni C, et al. 1997. J. Clin. Invest. 100:1137.
3. Sallusto F, et al. 1997. Science. 277:2005.
4. Loetscher P, et al. 2001. J. Biol. Chem. 276:2986.
- Upregulated by high affinity Fc IgE receptor ligation (mast cells), RANTES (keratinocytes), IFNg (monocytes, neutrophils), HIV tat protein (basophils), IL-3, IL-5 and GM-CSF (CD34+ progenitor cells), IL-2 and IL-4(T cells). Downregulated by IL-
- Gene ID
- 1232 View all products for this Gene ID
- View information about CD193 on UniProt.org
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
|Purified anti-human CD193 (CCR3)||5E8||FC, IHC-P|
|PE anti-human CD193 (CCR3)||5E8||FC|
|Brilliant Violet 605™ anti-human CD193 (CCR3)||5E8||FC|
|APC anti-human CD193 (CCR3)||5E8||FC|
|Alexa Fluor® 647 anti-human CD193 (CCR3)||5E8||FC|
|APC/Cyanine7 anti-human CD193 (CCR3)||5E8||FC|
|Brilliant Violet 421™ anti-human CD193 (CCR3)||5E8||FC|
|PerCP/Cyanine5.5 anti-human CD193 (CCR3)||5E8||FC|
|FITC anti-human CD193 (CCR3)||5E8||FC|
|Brilliant Violet 510™ anti-human CD193 (CCR3)||5E8||FC|
|Biotin anti-human CD193 (CCR3)||5E8||FC|
|APC/Fire™ 750 anti-human CD193 (CCR3)||5E8||FC|
|PE/Dazzle™ 594 anti-human CD193 (CCR3)||5E8||FC|
|TotalSeq™-A0397 anti-human CD193 (CCR3)||5E8||PG|
|TotalSeq™-C0397 anti-human CD193 (CCR3)||5E8||PG|
|Brilliant Violet 711™ anti-human CD193 (CCR3) Antibody||5E8||FC|
|TotalSeq™-D0397 anti-human CD193 (CCR3)||5E8||PG|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human CD193 (CCR3)
PE anti-human CD193 (CCR3)
Brilliant Violet 605™ anti-human CD193 (CCR3)
APC anti-human CD193 (CCR3)
Alexa Fluor® 647 anti-human CD193 (CCR3)
APC/Cyanine7 anti-human CD193 (CCR3)
Brilliant Violet 421™ anti-human CD193 (CCR3)
PerCP/Cyanine5.5 anti-human CD193 (CCR3)
FITC anti-human CD193 (CCR3)
Brilliant Violet 510™ anti-human CD193 (CCR3)
Biotin anti-human CD193 (CCR3)
APC/Fire™ 750 anti-human CD193 (CCR3)
PE/Dazzle™ 594 anti-human CD193 (CCR3)
TotalSeq™-A0397 anti-human CD193 (CCR3)
TotalSeq™-C0397 anti-human CD193 (CCR3)
Brilliant Violet 711™ anti-human CD193 (CCR3) Antibody
TotalSeq™-D0397 anti-human CD193 (CCR3)