PE/Cyanine7 anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
TER119_PECy7_020808
C57BL/6 mouse bone marrow cells stained with TER119 PE/Cyanine7
  • TER119_PECy7_020808
    C57BL/6 mouse bone marrow cells stained with TER119 PE/Cyanine7
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
116221 25 µg 68,00€
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116222 100 µg 156,00€
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  2. Fu X, et al. 2015. J Biol Chem. 290: 26445 - 26456. PubMed
  3. Xie X, et al. 2015. Sci Rep. 5: 18115. PubMed
  4. Maimets M, et al. 2016. Stem Cell Reports. 6:150-162. PubMed
  5. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  6. van Gastel N, et al. 2020. Cell Metabolism. 32(3):391-403.e6. PubMed
  7. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  8. Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed
  9. Silva C, et al. 2019. Cell Physiol Biochem. 52:503. PubMed
  10. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  11. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  12. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  13. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  14. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  15. Sun C, et al. 2020. Cell Stem Cell. 26(5):707-721. PubMed
  16. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
RRID
AB_2137789 (BioLegend Cat. No. 116221)
AB_2281408 (BioLegend Cat. No. 116222)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All TER-119 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TER-119/Erythroid Cells TER-119 FC
Biotin anti-mouse TER-119/Erythroid Cells TER-119 FC
FITC anti-mouse TER-119/Erythroid Cells TER-119 FC
PE anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody TER-119 FC
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells TER-119 FC, IHC-F, IHC-P, IP, WB
Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells TER-119 FC
Pacific Blue™ anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells TER-119 FC, IHC-F, IHC-P, IP, WB, CMCD
Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready) TER-119 FC, CyTOF®
APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
Go To Top Version: 2    Revision Date: 12-04-2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse TER-119/Erythroid Cells

  • Biotin anti-mouse TER-119/Erythroid Cells

  • FITC anti-mouse TER-119/Erythroid Cells

  • PE anti-mouse TER-119/Erythroid Cells

  • Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells

  • Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells

  • APC/Cyanine7 anti-mouse TER-119/Erythroid Cells

  • Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells

  • APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells

  • Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells

  • PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells

  • TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells

  • Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells

  • Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells

  • Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody

  • PE/Cyanine5 anti-mouse TER-119/Erythroid Cells

  • Purified anti-mouse TER-119/Erythroid Cells

  • Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells

  • Pacific Blue™ anti-mouse TER-119/Erythroid Cells

  • TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells

  • Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells

  • Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells

  • Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells

  • PerCP anti-mouse TER-119/Erythroid Cells

  • PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells

  • Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready)

  • APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells

  • TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells

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