PE/Cyanine7 anti-mouse/human KLRG1 (MAFA) Antibody

Pricing & Availability
Clone
2F1/KLRG1 (See other available formats)
Regulatory Status
RUO
Other Names
MAFA, 2F1-Ag
Isotype
Syrian hamster IgG
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Product Citations
publications
2F1.KLRG1_PECy7_KLRG1_Antibody_FC_121012
C57BL/6 mouse splenocytes was stained with NK1.1 APC and KLRG1 (clone 2F1/KLRG1) PE/Cyanine7.
  • 2F1.KLRG1_PECy7_KLRG1_Antibody_FC_121012
    C57BL/6 mouse splenocytes was stained with NK1.1 APC and KLRG1 (clone 2F1/KLRG1) PE/Cyanine7.
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
138415 25 µg 84€
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138416 100 µg 220€
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Description

Killer cell lectin-like receptor G1 (KLRG1) is the mouse homolog of the rat mast cell function-associated antigen (MAFA or 2F1-Ag).  KLRG1 is a type II membrane glycoprotein that was first identified on the surface of rat mast cell line RBL-2H3.  It is composed of a homodimer of glycosylated 30-38 kD subunits.  Mouse and human homologs of KLRG1 are expressed by subsets of NK cells and lymphokine-activated killer (LAK) cells but not mast cells.  KLRG1 is also expressed on subsets of CD8+ and CD4+ cells, including CD4+ and CD8+ effector/memory cells, potent regulatory CD4+ T cells.  KLRG1 may be involved in regulating NK cell homeostasis.  KLRG1 was found to recognize cadherins and thus inhibit immune responses by regulating the effector function and the developmental processes of NK and T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
IL-2 activated NK cells from C57BL/6 mice
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

For successful staining of human cells, brighter fluorophore-antibody conjugates, such as PE, BV421™, or APC, are recommended.

Additional reported applications (for relevant formats) include: Western Blotting1.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Robbins SH, et al. 2002. J. Immunol. 168:2585. (WB)
  2. Robbins SH, et al. 2003. J. Immunol. 170:5876. (FC)
  3. McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC)
Product Citations
  1. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  2. Beura LK, et al. 2018. Immunity. 48:327. PubMed
  3. Ahrends T, et al. 2017. Immunity. 47:848. PubMed
  4. Li C, et al. 2018. Cell. 174:285. PubMed
  5. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  6. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  7. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  8. Gagnon JD, et al. 2019. Cell Rep. 28:2169. PubMed
  9. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  10. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  11. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
  12. Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
  13. Bharath LP, et al. 2020. Cell Metabolism. 32(1):44-55.e6.. PubMed
  14. He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
  15. Alwis R, et al. 2016. J Virol. 90: 4771 - 4779. PubMed
  16. Joosten S, et al. 2016. PLoS Pathog. 12: 1005687. PubMed
  17. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  18. Jaiswal AK, et al. 2020. Immunobiology. 225:151956. PubMed
  19. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  20. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  21. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
RRID
AB_2561735 (BioLegend Cat. No. 138415)
AB_2561736 (BioLegend Cat. No. 138416)
Disclaimer

This product may be used for research purposes only.  It is not licensed for resale and may only be used by the buyer.  This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service.  If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California.  The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
KLRG1 is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM, expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kD.
Distribution

Subsets of NK cells, lymphokine-activated killer (LAK) cells and subsets of CD8+ and CD4+ cells.

Function
Regulates NK cell proliferation, maturation, and homeostasis. Inhibits immune responses by regulating the effector function and the developmental processes of NK and T cells.
Ligand/Receptor
E-cadherin
Cell Type
NK cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Antigen References

1. Grundemann C, et al. 2006. J. Immunol. 176:1311.
2. Blaser C, et al. 1998. J. Immunol. 161:6451.
3. Huntington ND, et al. 2007. J. Immunol. 178:4764.
4. Voehringer D, et al. 2001. J. Immunol. 167:4834.

Gene ID
50928 View all products for this Gene ID
UniProt
View information about KLRG1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02-11-2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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