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PE/Cyanine7 anti-mouse CD357 (GITR) Antibody

Pricing & Availability
Clone
DTA-1 (See other available formats)
Regulatory Status
RUO
Other Names
Glucocorticoid-induced TNFR -related gene, TNFRSF18
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
DTA-1_PECy7_CD357_Antibody_FC_041714
C57BL/6 mouse splenocytes were stained with CD357 (clone DTA-1) PE/Cyanine7 (filled histogram) or rat IgG2b PE/Cyanine7 isotype control (open histogram).
  • DTA-1_PECy7_CD357_Antibody_FC_041714
    C57BL/6 mouse splenocytes were stained with CD357 (clone DTA-1) PE/Cyanine7 (filled histogram) or rat IgG2b PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
126317 25 µg 118€
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126318 100 µg 268€
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Description

GITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF receptor superfamily, also known as TNFRSF18 and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD25+ CD4+ Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-κB activation, and abrogate the inhibitory function of CD25+ CD4+ Tregs. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD25+ CD4+ T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References
  1. De Luca A, et al. 2007. J. Immunol. 179:5999. PubMed
Product Citations
  1. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  2. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  3. Araujo Furlan CL, et al. 2018. Front Immunol. 2.149305556. PubMed
  4. Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
  5. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  6. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
RRID
AB_2563385 (BioLegend Cat. No. 126317)
AB_2563386 (BioLegend Cat. No. 126318)

Antigen Details

Structure
66-70 kD homodimer of TNFR superfamily member.
Distribution

Low levels on resting T cells, B cells, and macrophages; high levels on CD25+ CD4+ Tregs and CD25+ CD4+ CD8- thymocytes.

Function
Stimulation of GITR can abrogate or reduce the inhibitory function of CD25+ CD4+ Treg cells. In vivo activation of GITR may lead to the development of autoimmune diseases.
Ligand/Receptor
GITR ligand (GITRL).
Cell Type
B cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059.
2. Shimizu J, et al. 2002 Nat. Immunol. 3:135.
3. Stephens GL, et al. 2004. J. Immunol. 173:5008.
4. McHugh RS, et al. 2002. Immunity 16:311.

Regulation
Activation of T and B cells can upregulate GITR expression.
Gene ID
21936 View all products for this Gene ID
UniProt
View information about CD357 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10-29-2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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