- JES6-5H4 (See other available formats)
- Regulatory Status
- Other Names
- Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
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IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- E. coli-expressed, recombinant mouse IL-2
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
ELISA Detection1-3 or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 & 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard.
Flow Cytometry8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations.
Neutralization1,7: The Ultra-LEAF™ purified antibody (Endotoxin in vivo and in vitro (Cat. No. 503845-503850)) is recommended for neutralization.
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections2, in vivo capture7, and immunocytochemistry.
Note: For testing mouse IL-2 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. No. 431001 & 431004) are specially developed and recommended.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
- Application References
- Abrams J, et al. 1992. Immunol. Rev. 127:5.
- Sander B, et al. 1993. J. Immunol. Meth. 166:201.
- Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20.
- Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19.
- Mo X, et al. 1995. J. Virol. 69:1288.
- Karulin A, et al. 2000. J. Immunol. 164:1862.
- Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
- Ko SY, et al. 2005. J. Immunol. 175:3309. PubMed
- Kang SS and Allen PM. 2005. J. Immunol. 174:5382.
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Product Citations
AB_2123674 (BioLegend Cat. No. 503824)
- Cytokine; 15-30 kD (Mammalian)
- Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
- Cell Sources
- T cells
- Cell Targets
- T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
- High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
- Cell Type
- Biology Area
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.
- Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
- Gene ID
- 16183 View all products for this Gene ID
- View information about IL-2 on UniProt.org
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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APC anti-mouse IL-2
Biotin anti-mouse IL-2
FITC anti-mouse IL-2
PE anti-mouse IL-2
Purified anti-mouse IL-2
Alexa Fluor® 488 anti-mouse IL-2
Alexa Fluor® 647 anti-mouse IL-2
Alexa Fluor® 700 anti-mouse IL-2
Pacific Blue™ anti-mouse IL-2
PerCP/Cyanine5.5 anti-mouse IL-2
PE/Cyanine5 anti-mouse IL-2
Brilliant Violet 421™ anti-mouse IL-2
Brilliant Violet 605™ anti-mouse IL-2
PE/Cyanine7 anti-mouse IL-2
Brilliant Violet 510™ anti-mouse IL-2
Purified anti-mouse IL-2 (Maxpar® Ready)
Brilliant Violet 711™ anti-mouse IL-2
PE/Dazzle™ 594 anti-mouse IL-2
APC/Fire™ 750 anti-mouse IL-2
Brilliant Violet 785™ anti-mouse IL-2
Spark Red™ 718 anti-mouse IL-2