Loading...

PE anti-rat CD200 Antibody

Pricing & Availability
Clone
OX-2 (See other available formats)
Regulatory Status
RUO
Other Names
OX-2, OX2
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
A_OX-2_PE_082509
LOU rat splenocytes stained with OX-2 PE and RT1D (OX-17) FITC
  • A_OX-2_PE_082509
    LOU rat splenocytes stained with OX-2 PE and RT1D (OX-17) FITC
  • B_OX-2_PE_082509
    LOU rat splenocytes double stained with mouse IgG1 (MOPC-21) PE isotype control and RT1D (OX-17) FITC
See PE spectral data
Cat # Size Price Quantity Check Availability Save
204807 100 µg 172€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD200, known as OX-2, is a type I membrane glycoprotein member of the Ig supergene family. CD200 is expressed on B cells, a subset of T cells, thymocytes, follicular dendritic cells, neurons, keratinocytes, vascular endothelium, and some smooth muscle. The interaction of CD200 with CD200 receptor provide a potent costimulatory T-cell signal in the presence of TCR signaling, stimulate macrophages, and inhibit mast cell degranulation. It was reported that increased expression of OX-2 on DC was associated with inhibition of cytokine production and renal allograft rejection. Incubation of lymphocytes with OX-2 Fc inhibits a primary mixed lymphocyte reaction in vitro, decreased IL-2 and IFN-γ production, increased IL-4 and IL-10 production. In vivo infusion of OX-2 Fc promotes both skin and renal graft survival and decreases the antibody response. The OX-2 antibody reacts with rat OX-2 antigen.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

1. Dick, A.D., et al. 2001. Invest Ophthalmol. Vis. Sci. 42:170.

RRID
AB_2073956 (BioLegend Cat. No. 204807)

Antigen Details

Structure
A 47kD (thymus) or 41-kD (brain) type I membrane glycoprotein member of the Ig supergene family. CD200 molecule contains two extracellular Ig-like domains, an Ig V-like, and an Ig C-like domain.
Distribution

CD200 is expressed on B cells, a subset of T cells, thymocytes, follicular dendritic cells, neurons, vascular endothelium, and some smooth muscle.

Function
The interaction of CD200 with CD200 receptor provide a potent costimulatory T-cell signal in the presence of TCR signaling, stimulate macrophages, and inhibit mast cell degranulation.
Ligand/Receptor
CD200 receptor
Cell Type
B cells, Dendritic cells, Endothelial cells, Neurons, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. McMaster WR, et al. 1979. Eur. J. Immunol. 9:426
2. Barclay A. N, et al. 1981. Immunology. 44:727
3. Bukovsky A, et al. 1984. Immunol. 52:631
4. Borriello F, et al. 1997. J. Immunol. 158:4548
5. Gorczynski R. M, et al. 1999. J. Immunol. 163:1654
6. Gorczynski L, et al. 1999. J. Immunol. 162:774

Gene ID
24560 View all products for this Gene ID
UniProt
View information about CD200 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account