PE anti-mouse CD206 (MMR) Antibody

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
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Product Citations
publications
C068C2_PE_1_082911
Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with CD206 (clone C068C2) PE (top) or rat IgG2a, κ PE isotype control (bottom).
  • C068C2_PE_1_082911
    Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with CD206 (clone C068C2) PE (top) or rat IgG2a, κ PE isotype control (bottom).
  • C068C2_PE_2_082911
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141705 25 µg 104€
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141706 100 µg 240€
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Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References
  1. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Feng Y, et al. 2018. J Am Soc Nephrol. 29:182. PubMed
  3. Seo JB, et al. 2019. Nat Metab. 1:86. PubMed
  4. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  5. Sun H, et al. 2019. Mol Hum Reprod. 25:05:00. PubMed
  6. Xiong Y, et al. 2020. J Nanobiotechnology. 18:66. PubMed
  7. Cui J, et al. 2021. J Cell Mol Med. 25:5586. PubMed
  8. Zhang B, et al. 2022. Front Med (Lausanne). 8:796743. PubMed
  9. Wang HH, et al. 2022. J Biomed Sci. 29:36. PubMed
  10. Zhang Z, et al. 2022. Front Pharmacol. 13:906625. PubMed
  11. Hu S, et al. 2018. Front Immunol. 9:01. PubMed
  12. Ying W et al. 2017. Cell. 171(2):372-384 . PubMed
  13. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  14. Clarke F,et al. 2017. PLoS One. 10.1371/journal.pone.0186625. PubMed
  15. Zhang Y, et al. 2018. Sci Rep. 8:5994. PubMed
  16. Hsu SPC, et al. 2020. J Neurooncol. 146:417. PubMed
  17. Sadek J, et al. 2021. EMBO Mol Med. 13:e13591. PubMed
  18. Li Q, et al. 2021. Front Immunol. 12:707784. PubMed
  19. Nomura M, et al. 2016. Nat Immunol. 17:216-217. PubMed
  20. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  21. Zheng W, et al. 2021. Wellcome Open Research. 6:52. PubMed
  22. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  23. Dong L, et al. 2021. Cancer Cell. . PubMed
  24. De Vlaeminck Y, et al. 2020. Cancers (Basel). 12:. PubMed
  25. Li J, et al. 2021. Front Immunol. 12:649285. PubMed
  26. Krukowski K, et al. 2021. Sci Adv. 7:eabg6702. PubMed
  27. Pajarinen J, et al. 2021. J Biomed Mater Res A. 109:1512. PubMed
  28. Zheng H, et al. 2021. Frontiers in Cell and Developmental Biology. 9:641527. PubMed
  29. Shao H, et al. 2012. J Biol Chem. 287:35860. PubMed
  30. Mul Fedele ML, et al. 2020. Front Cell Infect Microbiol. 10:100. PubMed
  31. El–Achkar GA, et al. 2019. PLoS One. 14:e0216405. PubMed
  32. Zhang J, et al. 2021. J Leukoc Biol. 110:485. PubMed
  33. Tanner L, et al. 2022. Front Pharmacol. 13:899469. PubMed
  34. Hou H, et al. 2021. Front Cell Dev Biol. 9:737003. PubMed
  35. Johansen AZ, et al. 2022. Pharmaceutics. 14:. PubMed
  36. Jia X, et al. 2021. Front Cell Dev Biol. 9:685522. PubMed
  37. He C, et al. 2021. Oncoimmunology. 1897295:10. PubMed
  38. Tang TT, et al. 2020. Sci Adv. 6:eaaz0748. PubMed
  39. Giri J, et al. 2020. Cell Rep. 30:1923. PubMed
  40. Dan H, et al. 2020. Mol Oncol. 14:795. PubMed
  41. Kim JS, et al. 2020. Int J Oncol. 56:1405. PubMed
  42. McKee AM, et al. 2021. iScience. 24:103012. PubMed
  43. Zhang Y, et al. 2022. Nat Commun. 13:4553. PubMed
  44. Rao X, et al. 2022. Cell Death Dis. 13:891. PubMed
  45. Farini A, et al. 2020. Front Physiol. 0.738194444. PubMed
  46. An W et al. 2019. British journal of pharmacology. 176(5):699-710 . PubMed
  47. Li J, et al. 2021. Cell Commun Signal. 19:89. PubMed
  48. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  49. Jiao Y, et al. 2021. Crit Care. 25:356. PubMed
  50. Martins L, et al. 2020. Stem Cell Res Ther. 0.786805556. PubMed
  51. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  52. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  53. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  54. Lu X, et al. 2020. Sci Transl Med. 12:. PubMed
  55. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  56. Yu Y, et al. 2018. Mol Med Rep. 17:8212. PubMed
  57. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  58. Wang Y, et al. 2022. Cell Death Dis. 13:748. PubMed
  59. Golden TN, et al. 2022. Front Pharmacol. 12:761496. PubMed
  60. Cai C, et al. 2021. Sci Rep. 11:13386. PubMed
  61. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  62. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  63. Ruiz de Azua I, et al. 2017. J Clin Invest. 127:4148. PubMed
  64. Miura I, et al. 2021. Physiol Rep. 9:e14859. PubMed
  65. Li H, et al. 2021. Nat Commun. 12:7149. PubMed
  66. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  67. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  68. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  69. Chu SY, et al. 2019. Nat Commun. 10:1524. PubMed
  70. Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
  71. Shu Y, et al. 2020. Immunology. 160:345. PubMed
  72. Ying W, et al. 2016. Sci Rep. 6:20176. PubMed
  73. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  74. Qiu N, et al. 2021. J Nanobiotechnology. 19:428. PubMed
  75. Yang C, et al. 2022. Nat Commun. 13:4866. PubMed
  76. Lu X, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2358. PubMed
  77. Lu SW, et al. 2020. Nat Commun. 3.660416667. PubMed
  78. Anderson-Baucum E, et al. 2021. Cell Metab. 33:1883. PubMed
  79. Stevenson ER, et al. 2022. J Pharmacol Exp Ther. 382:356. PubMed
  80. Wang X, et al. 2022. J Biol Chem. 298:101561. PubMed
  81. Lowe PP, et al. 2020. J Neuroinflammation. 0.913888889. PubMed
  82. Zhou K, et al. 2016. J Cereb Blood Flow Metab. 10.1177/0271678X16648712. PubMed
  83. Deng W, et al. 2015. Clin Immunol. . PubMed
  84. Long L, et al. 2018. Bio Protoc. 8:e2815. PubMed
  85. Li C, et al. 2019. JCI Insight. 5. PubMed
  86. Budai Z, et al. 2021. Cells. 10:. PubMed
  87. Zhu Q, et al. 2022. Atherosclerosis. 357:41. PubMed
  88. Asterholm I, et al. 2012. J Lipid Res. 53: 1254. PubMed
  89. Stridh L, et al. 2013. J Neurosci. 33:12401. PubMed
  90. Krukowski K, et al. 2018. Sci Rep. 8:7857. PubMed
  91. Di Lorenzo A, et al. 2022. Oncoimmunology. 11:2086752. PubMed
  92. Li Y, et al. 2020. Theranostics. 10:2897. PubMed
  93. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  94. Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed
  95. Suzuki T, et al. 2017. Cell Rep. 18(8):2045-2057. PubMed
  96. Ukah T, et al. 2017. J Immunol. 10.4049/jimmunol.1700410. PubMed
  97. Roediger B et al. 2018. Cell. 175(2):530-543 . PubMed
  98. Liu C, et al. 2022. Cell Death Discov. 8:269. PubMed
  99. Sears SM, et al. 2022. Kidney360. 3:818. PubMed
  100. Santopaolo M, et al. 2021. Front Immunol. 609406:12. PubMed
  101. Zhang J, et al. 2020. J Cell Mol Med. 24:4547. PubMed
  102. Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
  103. Liu YS, et al. 2021. Cancers (Basel). 13:. PubMed
  104. Budhu S, et al. 2021. Cell Reports. 34(2):108620. PubMed
  105. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  106. Browne A, et al. 2015. Cancer Res . 75: 2811 - 2821. PubMed
  107. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  108. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  109. Li B et al. 2019. Cell reports. 26(10):2720-2737 . PubMed
  110. Morioka S, et al. 2018. Nature. 563:714. PubMed
  111. Mellal K, et al. 2019. Sci Rep. 9:12903. PubMed
  112. Yuan Y, et al. 2019. Clin Sci (Lond). 133:1759. PubMed
  113. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  114. Al-Zaeed N, et al. 2021. Cell Death Dis. 12:611. PubMed
  115. Golabek A, et al. 2021. Exp Ther Med. 21:482. PubMed
  116. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  117. Xu GY, et al. 2022. Small. 18:e2107838. PubMed
  118. Chuan Li et al. 2017. Endocrinology. 158(9):2837-2847 . PubMed
  119. Wen HJ, et al. 2019. Cell Mol Gastroenterol Hepatol. 8:p173. PubMed
  120. Yuan M, et al. 2017. Hum Reprod. 32:94. PubMed
  121. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  122. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  123. Cai T, et al. 2022. Front Immunol. 13:1009932. PubMed
  124. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
  125. Guo S, et al. 2021. Chronic Dis Transl Med. 7:254. PubMed
  126. Zheng H, et al. 2021. Frontiers in Immunology. 12:645100. PubMed
RRID
AB_10896421 (BioLegend Cat. No. 141705)
AB_10895754 (BioLegend Cat. No. 141706)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Why is mouse CD206 stained intracellularly and not via surface staining?

Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.

Go To Top Version: 3    Revision Date: 04-04-2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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