PE anti-human TRA-1-60-R Antibody

Pricing & Availability
TRA-1-60-R (See other available formats)
Other Names
Podocalyxin, TRA-1
Mouse IgM, κ
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Product Citations
NCCIT (human teratocarcinoma cell line) stained with TRA-1-60-R PE (red line) or mouse IgM, κ PE isotype control (blue line).
  • Tra-1-60-R_PE_121508.jpg
    NCCIT (human teratocarcinoma cell line) stained with TRA-1-60-R PE (red line) or mouse IgM, κ PE isotype control (blue line).
See PE spectral data
Cat # Size Price Quantity Avail. Save
330609 25 tests 100€
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330610 100 tests 164€
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TRA-1-60-R antibody recognizes the neuraminidase resistant form of TRA-1-60. TRA-1-60 is a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-60 on human ESC is downregulated upon differentiation. The soluble form of TRA-1-60 has been found in the serum of patients with embryonic cancers. Historically, TRA-1-60 is a neuroaminidase-sensitive (TRA-1-60-S) carbohydrate epitope expressed on podocalyxin, a member of the mucin-like glycoprotein CD34 family. Podocalyxin is a type I transmembrane sialoprotein with anti-adhesion properties. Further studies have shown that some of TRA-1-60 is neuraminidase resistant (TRA-1-60-R).  The two forms of TRA-1-60 are essentially indistinguishable in terms of their cellular reactivity except for the difference in neuraminidase sensitivity.  Both of TRA-1-60-S and TRA-1-60-R appear to be the same molecular weight glycoproteins detected by Western blot.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
Human embryonal carinoma cell line 2102Ep cl.2A6
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, immunohistochemical staining, acetone-fixed frozen sections, immunoprecipitation, and radioimmunoassay.

Application References
  1. Miyoshi N, et. al. 2010. P. Natl. Acad. Sci. USA 107:40. (IF)
  2. Kuai XL, et al. 2009. Differentiation 77:229. (WB)
  3. King F, et al. 2009. Stem Cells Dev. 18:1441. (FC)
Product Citations
  1. Steenpass L 2017. Stem Cell Res.. 10.1016/j.scr.2017.07.005. PubMed
  2. Simic MS, et al. 2019. Sci Adv. 5:eaaw0025. PubMed
  3. Neureiter A et al. 2018. Stem Cell Res. 33:20-24 . PubMed
  4. Schipper L et al. 2018. Stem Cell Res. 33:41-45 . PubMed
  5. Kuang YL, et al. 2019. Stem Cell Res. 37:101434. PubMed
AB_1279447 (BioLegend Cat. No. 330609)
AB_2119065 (BioLegend Cat. No. 330610)

Antigen Details


Embryonic stemcells

Cell Type
Embryonic Stem Cells
Biology Area
Immunology, Stem Cells
Gene ID
5420 View all products for this Gene ID
View information about TRA-1-60-R on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11-30-2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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