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PE anti-human CD27 Antibody

Pricing & Availability
Clone
O323 (See other available formats)
Regulatory Status
RUO
Workshop
IV T-186
Other Names
S152, T14, TNFRSF7
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O323_PE_020808
Human peripheral blood lymphocytes stained with O323 PE
  • O323_PE_020808
    Human peripheral blood lymphocytes stained with O323 PE
  • O323_PE_CD27_Antibody_04292022
    PE anti-human CD27 Antibody Human peripheral blood was stained with CD27 (clone O323) PE (solid line) or mouse IgG1, κ PE isotype control (dashed line).

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
302807 25 tests 36€
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302808 100 tests 84€
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302842 100 µg 94€
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Description

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  2. Chen YQ et al. 2018. Cell. 173(2):417-429 . PubMed
  3. Chen F, et al. 2021. Immunity. 54(4):781-796.e4. PubMed
  4. Swanson E, et al. 2021. eLife. 10:00. PubMed
  5. Soriano-Sarabia N, et al. 2014. J Virol. 88:14070. PubMed
  6. Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed
  7. Savage AK, et al. 2021. iScience. 24(5):102404. PubMed
  8. Wong GK, et al. 2019. JCI Insight. 4. PubMed
  9. Genge PC, et al. 2021. STAR Protoc. 2:100900. PubMed
  10. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  11. Li X, et al. 2021. Front Immunol. 12:779560. PubMed
RRID
AB_314299 (BioLegend Cat. No. 302807)
AB_314300 (BioLegend Cat. No. 302808)
AB_2564146 (BioLegend Cat. No. 302842)

Antigen Details

Structure
TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution

Medullary thymocytes, T and B cell subsets, NK cells

Function
T cell costimulation
Ligand/Receptor
CD70
Cell Type
B cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hintzen R, et al. 1994. Immunol. Today 15:307.
2. Agematsu K, et al. 1995. J. Immunol. 154:3627.

Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11-30-2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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