PE anti-human CD18 Antibody

Pricing & Availability
Clone
1B4/CD18 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin beta-2, integrin beta chain, beta 2
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1B4slashCD18_PE_CD18_Antibody_082517
Human peripheral blood lymphocytes were stained with CD18 (clone 1B4/CD18) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
  • 1B4slashCD18_PE_CD18_Antibody_082517
    Human peripheral blood lymphocytes were stained with CD18 (clone 1B4/CD18) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
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Cat # Size Price Quantity Check Availability Save
373407 25 tests 80€
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373408 100 tests 199€
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Description

CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral blood lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 1B4/CD18 antibody was able to partially block the Ts1/18 antibody, indicating the two anti-CD18 antibodies recognize distinct but overlapping epitopes. 

Application References
  1. Singer II, et al. 1993. J. Immunol. 150:2844.
  2. Seth R, et al. 1991. FEBS Lett. 282:193.
Product Citations
  1. Bouafia A, et al. 2018. J Clin Invest. 129:1047. PubMed
  2. Velázquez–Avila M, et al. 2019. Leukemia. 33:1337. PubMed
RRID
AB_2716023 (BioLegend Cat. No. 373407)
AB_2716024 (BioLegend Cat. No. 373408)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein
Distribution

Leukocytes

Function
Adhesion and signaling in hematopoietic cells
Interaction
Associates with CD11a, CD11b, or CD11c
Ligand/Receptor
ICAMs
Cell Type
Leukocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hogg N, et al. 1999. J. Clin. Invest. 103:97.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Springer TA. 1994. Cell 76:301.

Gene ID
3689 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 08-29-2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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