Pacific Blue™ anti-mouse Lineage Cocktail with Isotype Ctrl

Pricing & Availability
17A2 ; RB6-8C5 ; RA3-6B2 ; Ter-119 ; M1/70 ;
Regulatory Status
Other Names
Rat IgG2a/Rat IgG2b
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Product Citations
C57BL/6 bone marrow cells were stained with CD117 FITC and Pacific Blue™ lineage cocktail (top plot) or Pacific Blue™ isotype control cocktail (bottom plot).
  • Lineage_Cocktail_PB_1_112811
    C57BL/6 bone marrow cells were stained with CD117 FITC and Pacific Blue™ lineage cocktail (top plot) or Pacific Blue™ isotype control cocktail (bottom plot).
  • Lineage_Cocktail_PB_2_112811
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133305 25 tests 200,00€
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133306 100 tests 400,00€
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The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The Pacific Blue™ Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3, clone 17A2; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. Pacific Blue™ Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.


Materials provided:
1. Pacific Blue™ anti-mCD3/ Pacific Blue™ anti-mLy-6G(Ly-6C)/ Pacific Blue™ anti-mCD11b/ Pacific Blue™ anti-mCD45R(B220)/ Pacific Blue™ anti-mTer-119
2. Pacific Blue™ Rat IgG2b/Rat IgG2a isotype controls

Materials not provided:
Cell Staining Buffer (Cat. No. 420201)

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
This two-reagent set contains Pacific Blue™ conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
The antibodies are purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Storage & Handling
The antibody solutions should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Garama D, et al. 2015. Mol Cell Biol. 35: 3646 - 3656. PubMed
  2. Grayson MH, et al. 2018. Front Immunol. 9:1587. PubMed
  3. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
AB_11126168 (BioLegend Cat. No. 133305)

Antigen Details


T cells, B cells, monocytes/macrophages, granulocytes, NK cells, and erythrocytes

Cell Type
B cells, Erythrocytes, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area
Antigen References

1. Morrison SJ, et al. 1997. Development 124:1929.
2. Okada S, et al. 1992. Blood 80:3044.
3. Spangrude GJ, et al. 1988. Science 241:58.
4. Spangrude GJ, et al. 1990. Exp. Hematol. 18:920.
5. Tan SL, et al. 2006. J. Immunol. 176:2872.

Gene ID
104231 View all products for this Gene ID
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Go To Top Version: 3    Revision Date: 07-09-2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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