Pacific Blue™ anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
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Product Citations
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A20_Blue_020608
SJL mouse splenocytes stained with A20 Pacific Blue™
  • A20_Blue_020608
    SJL mouse splenocytes stained with A20 Pacific Blue™
See Pacific Blue™ spectral data
Cat # Size Price Quantity Avail. Save
110721 25 µg 68€
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110722 100 µg 156€
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Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Hamilton J,et al. 2017. J Immunol. 10.4049/jimmunol.1700888. PubMed
  2. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  3. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  4. Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed
  5. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  6. Wünsche P et al. 2018. Cell stem cell. 23(1):132-146 . PubMed
  7. Jing Li et al. 2018. Immunity. 48(4):773-786 . PubMed
  8. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  9. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  10. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  11. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  12. Marcandalli J et al. 2019. Cell. 176(6):1420-1431 . PubMed
  13. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  14. Page N, et al. 2018. Immunity. 48:937. PubMed
  15. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  16. Fiege JK, et al. 2019. J Immunol. 203(4):936-945. PubMed
  17. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  18. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  19. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  20. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  21. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  22. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  23. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  24. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  25. Holian C 2007. Am J Respir Cell Mol Biol. 37:729. PubMed
  26. Li L, et al. 2010. J Immunol. 184:1728. PubMed
  27. Lin G, et al. 2013. J Immunol. 190:4627. PubMed
  28. Mills R, et al. 2015. J Immunol. 194:3675. PubMed
  29. Nadrah K, et al. 2015. PLoS One. 10: 0136061. PubMed
  30. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  31. Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed
  32. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
  33. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  34. Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed
  35. Wigton E, et al. 2016. J Leukoc Biol. 100: 143 - 153. PubMed
  36. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  37. Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed
RRID
AB_492867 (BioLegend Cat. No. 110721)
AB_492866 (BioLegend Cat. No. 110722)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11-30-2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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