Pacific Blue™ anti-human HLA-A2 Antibody

Pricing & Availability
BB7.2 (See other available formats)
Regulatory Status
Other Names
MHC-class I
Mouse IgG2b, κ
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Product Citations
Peripheral blood lymphocytes from HLA-A2 positive (top) and HLA-A2 negative (bottom) donors were stained with anti-human HLA-A2 (clone BB7.2) Pacific Blue™ (filled histograms) or mouse IgG2b Pacific Blue™ isotype control (open histograms).
  • BB7.2_PB_HLA-A2_Antibody_FC_1_092812
    Peripheral blood lymphocytes from HLA-A2 positive (top) and HLA-A2 negative (bottom) donors were stained with anti-human HLA-A2 (clone BB7.2) Pacific Blue™ (filled histograms) or mouse IgG2b Pacific Blue™ isotype control (open histograms).
  • BB7.2_PB_HLA-A2_Antibody_FC_2_092812
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
343312 100 tests 268€
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HLA-A2 is most common in Northern Asia and North America populations. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells, playing an important role in cell-mediated immune responses and tumor surveillance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Papain solubilized HLA-A2
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

The BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by A*02 alleles.

Additional reported applications (for the relevant formats) include: immunoprecipitation3.

Application References
  1. Brodsky FM, et al. 1979. Immunol. Rev. 47:3.
  2. Parham P and Brodsky FM. et al. 1981. Hum. Immunol. 3:277.
  3. Lubben NB, et al. 2007. Mol Biol Cell. 18:3351. (IP)
Product Citations
  1. Massafra V, et al. 2021. J Immunol. 207:493. PubMed
  2. Mo F, et al. 2021. Nat Biotechnol. 56:39. PubMed
AB_2561569 (BioLegend Cat. No. 343312)

Antigen Details

Ig superfamily

A*02 individuals leukocytes

Antigen presentation
Cell Type
Biology Area
Molecular Family
MHC Antigens
Gene ID
3105 View all products for this Gene ID
View information about HLA-A2 on

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Go To Top Version: 2    Revision Date: 02-15-2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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