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Mouse Regulatory B Cell (B10) Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor® 647)

Pricing & Availability
Clone
6D5; 53-7.3; 1B1;
Regulatory Status
RUO
Other Names
Breg, B regulatory
Isotype
Rat IgG2a/Rat IgG2b
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A_B10FlowKit_050109.jpg
C57BL/6 mouse splenocytes were stained with Mouse Regulatory B Cell Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor® 647). Histogram shows staining with CD19 FITC; dot plot data shows staining with CD1d Alexa Fluor® 647 and CD5 PE on gated CD19+ cells.
  • A_B10FlowKit_050109.jpg
    C57BL/6 mouse splenocytes were stained with Mouse Regulatory B Cell Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor® 647). Histogram shows staining with CD19 FITC; dot plot data shows staining with CD1d Alexa Fluor® 647 and CD5 PE on gated CD19+ cells.
  • z6D5_53-7.3_1B1_061410
    C57BL/6 mouse splenocytes were stained with Mouse Regulatory B Cell Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor® 647). Histogram shows staining with CD19 FITC; dot plot data shows staining with CD1d Alexa Fluor® 647 and CD5 PE on gated CD19+ cells.
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132601 25 tests 278€
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Description

Splenic IL-10-producing CD1dhighCD5+ B cells have been designated as regulatory B cells (Breg or B10). These rare IL-10- producing B cells represent only 1%-2% of spleen B cells and 7%-8% of peritoneal B cells in mice but are not normally detectable in blood or lymph nodes. B10 cells are a unique subset of potent regulatory B cells different from B-1a, B-1b, or CD21+CD23- mantle zone B cells. B10 cells produce IL-10 and negatively regulate T cell function. BioLegend's Mouse Regulatory B Cell Flow Kit is composed of fluorochrome conjugated anti-mouse CD19, CD5, and CD1d. It is optimized for detection or isolation of B10 cells by flow cytometry.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • FITC anti-mouse CD19/PE anti-mouse CD5/Alexa Fluor® 647 anti-mouse CD1d, 25 tests
  • FITC Rat IgG2a/PE Rat IgG2a/Alexa Fluor® 647 Rat IgG2b isotype controls, 25 tests

Product Details

Verified Reactivity
Mouse
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

This product has a shelf-life of 12 months or less. Please contact our technical support team for lot specific CoA and expiration date inquiries of this product.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells.

Materials Not Included
  • Cell Staining Buffer (Cat. No. 420201)
  • TruStain FcX (Cat. No. 101319)
  • Single color compensation controls

Antigen Details

Distribution

Represent 1%-2% of spleen B cells in wild-type mice, but expand to about 10% of spleen B cells in hCD19Tg mice.

Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

Yanaba K, et al. 2008. Immunity 25:639.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 12-12-2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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