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Mouse CD11b+ cells are either selected or depleted by incubating the sample with a biotin conjugated anti-mouse CD11b+ antibody and then magnetic streptavidin nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD11b+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
- Kit Contents
For Cat. No. 480109:
- 1 vial containing 200 µL of biotin anti-mouse CD11b+ antibody (clone M1/70)
- 1 vial containing 200 µL of streptavidin nanobeads
For Cat. No. 480118:
- 2 vials containing 1 mL each of biotin anti-mouse CD11b+ antibody (clone M1/70)
- 2 vials containing 1 mL each of streptavidin nanobeads
- Verified Reactivity
Antibody: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2 with stabilizer.
Streptavidin nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
The antibodies were purified by affinity chromatography, and conjugated wtih biotin under optimal conditions.
Streptavidin nanobeads: Protein-coated magnetic beads.
- Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
Volume of biotin antibody and streptavidin nanobeads should be adjusted depending on the different tissue of CD11b+ cells to be isolated. Use the table below as an example when working with different mouse tissue. For 1x107 cells in 100 µL of buffer, use the following volumes:
Optimal Nanobeads Volume
Mouse Bone Marrow
The volumes indicated in the table are for the use of MojoSort™ magnet (Cat. Nos. 480109 & 480110). For low frequency cells, pre-dilute the streptavidin nanobeads in order to pipette a minimum of 5 µL of any solution. Avoid working with small volumes.
- Application Notes
This kit is designed for the positive selection or depletion of mouse CD11b+ cells from mouse bone marrow, lymphoid tissue, lung and brain, among other organs.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
This kit is not recommended for CD11b positive selection from human samples based on in-house testing results.
Antibody or cocktail dilution to use in column: 32X
Nanobead dilution to use in columns: 5X
- Is there a way to detach your magnetic particles from the cell surface?
No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
The average diameter is approximately 130 nm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
Please contact our technical service team for further assistance.