LEGENDScreen™ Mouse PE Kit

Product Details

Verified Reactivity

Mouse

Formulation

Lyophilized antibodies in 96-well plate

Storage & Handling

Store unopened kit components at 2 - 8°C. Do not use this kit beyond its expiration date. Once opened, reconstitute plates with distilled, deionized water. The reconstituted plates can be used immediately or sealed for storage at 2 - 8°C in the dark for up to one month. Keep the buffer(s) at 2 - 8°C and use within one month after opening.

Application

Cell Screening

Application Notes

Materials Provided in Kit:
3 Mouse Cell Screening Plates containing:
 · 253 PE-conjugated Monoclonal Antibodies
 · 11 Mouse, Rat, or Hamster Ig Isotype Controls
Cell Staining Buffer (Cat. No. 420201)
Fixation Buffer (Cat. No. 420801)
Plate Sealers (8 sheets)

Additional Product Notes

 Download the Excel Version of the Specificity/Clone List

Antigen Details

Biology Area

Immunology

Molecular Family

CD Molecules

Gene ID

NA

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

If I don't have enough cells and use much less than 4 million/mL (~ 3 x 10⁵ cells/well), will it still work?

This may work with lower numbers of total cells, but we recommend trying to keep higher concentrations of cells for faster analysis. Of course, how many cells are needed depends on the specific application. Successful staining has been done with 1 x 10⁵ cells/well.

What are the guarantees regarding the lyophilized plate compared to the reconstituted plate?

Lyophilized product has a guaranteed shelf life of 6 months unopened. Reconstituted plates can be used or stored at 2 - 8°C sealed in the dark and used within a month. Be sure to properly seal the plates to prevent evaporation and shield the antibodies from light.

How should the kit be stored?

The kit should be stored at 2 - 8°C upon receipt. Once opened, the plates must be reconstituted immediately. Reconstituted plates can be used or stored at 2 - 8°C sealed in the dark and used within a month.

I have added my own antibody solution to the lyophilized product, will the lyophilized antibody work?

Yes, as long as the fluorophores on these antibodies are compatible and proper compensation has been applied during acquisition and analysis.

I am not going to use all the reconstituted antibody solution. Can I keep the left over for later or re-dry the solution in dark?

The antibody is in a one test per well format. There will not be any antibody left if the full test is used. Customers may decide to use less than the recommended volume per test, but this is not recommended and the performance is not guaranteed. Customers may also selectively transfer certain antibodies from the original plate to a new plate and use after reconstitution. If any antibody is not used after reconstitution, the plate can be sealed and store at 2 - 8°C for a month in the dark. Once reconstituted, re-drying is not recommended as this may result in a loss of signal.

What type of PE do you use in your conjugates?

We use R-PE in our conjugates.

Can I use my existing institution discount for this new product?

Yes.

Can I use half or less of the plate and keep the rest for later?

Yes. Customers can use half of the plate or whatever specificities they are interested in. However, the whole plate should be reconstituted. The half plate of antibodies must be transferred to another empty plate for the staining. The remaining half must be sealed and stored at 2 - 8°C in the dark and used within a month.

Are these plates made under sterile conditions?

The plates are not sterile. You would handle them as you would handle most typical flow cytometry staining protocols.

How do I request a custom LEGENDScreen™ product with only my specificities of interest?

For more info, visit: biolegend.com/custom_solutions.

What is the level of variability from one experiment to the other?

If the protocol is followed closely, the variability should be minimal. The variability should be similar to single vial antibody staining.

Concentration & Expiration Lookup

Certificate of Analysis