LEGEND MAX™ Mouse CXCL1 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Mouse CXCL1, keratinocytes-derived chemokine, KC, GRO1 oncogene, Melanoma Growth Stimulating Activity alpha, MGSA-alpha, CXCR2
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Product Citations
publications
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  • Mouse_CXCL1_ELISA_010721.png
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447507 1 Pre-coated Plate 304€
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Description

Mouse CXCL1 is a small peptide chemokine that belongs to the CXC subfamily. It was also previously known as KC, GRO1 oncogene, GRO alpha, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha). Mature mouse CXCL1 sequence contains 77 amino acids while human CXCL1 contains 73 amino acids. In addition, mouse CXCL1 is 60% identical to its human counterpart. A variety of cells including macrophages, T cells, and epithelial cells can produce and secrete the CXCL1 protein, which mediates its function through binding to glycosaminoglycans (GAGs) and the receptor CXCR2. Although CXCL1 has an important role in inflammatory responses by recruiting and activating neutrophils, aberrant expression of CXCL1 was also reported to associate with growth and progression of certain tumors such as breast, lung, and gastric cancers.

The LEGEND MAX™ Mouse CXCL1 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with anti-mouse CXCL1 polyclonal antibody. The detection antibody is a biotinylated anti-mouse CXCL1 polyclonal antibody. This kit is specifically designed for the accurate quantitation of mouse CXCL1 from serum and plasma. It is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse CXCL1 pre-coated 96-well Strip Microplate
  • Mosue CXCL1 Detection antibody
  • Mouse CXCL1 Standard
  • Avidin HRP
  • Assay Buffer B
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Mouse
Application

ELISA

Product Citations
  1. Ma X, et al. 2022. Cell Mol Immunol. . PubMed
Sensitivity
1.8 ± 0.5 pg/mL
Standard Range
15.6 – 1000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 1    Revision Date: 01-07-2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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