LEGEND MAX™ β-Amyloid x-40 ELISA Kit (Previously Covance catalog# SIG-38954)

Pricing & Availability
Other Names
Amyloid Beta A4 Protein, Prea4, Protease Nexin-II, Peptidase Nexin-II, Beta-Amyloid Peptide, Alzheimer Disease Amyloid Protein, Cerebral Vascular Amyloid Peptide, APP, Amyloid Precursor Protein
Covance Catalog# SIG-38954
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Product Citations
  • BetaMark-b-Amyloid-x40-TMB
Cat # Size Price Quantity Save
842301 1 Pre-coated Plate 460€
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Technical Data Sheet (pdf)

Kit Contents

Kit Contents

• Capture Antibody Coated Plate, 1 plate
• 1-40 Standard, 2 vials containing 20ug each 
• 5X Wash Buffer, 125mL
• Standard Diluent, 2 x 3mL
• 2X Incubation Buffer, 12mL
• HRP Detection Antibody, 40uL
• TMB Substrate, 22mL
• Plate Sealer, 2
• 1.5mL Microfuge tubes, 20

Product Details

Human, Mouse, Rat
Storage & Handling
Store between 2°C and 8°C.


Recommended Usage

Please download the Colorimetric LEGEND MAX™ ELISA Kit Protocol for further assay detail.

Application Notes

This antibody kit is used to quantify β-Amyloid [x-40].

This kit has a guaranteed shelf life of 2 to 6 months.

BioLegend's Colorimetric LEGEND MAX™ x-40 kit is specific for the x-40 isoform of beta amyloid. It has negligible cross reactivity with the x-42 isoform of beta amyloid. The Colorimetric LEGEND MAX™ 40 kit reacts with both human and rodent x-40.

Please note that the name of this kit has changed from BetaMark™ β-Amyloid x-40 ELISA Kit (TMB) to LEGEND MAX™ β-Amyloid x-40 ELISA Kit with Pre-coated Plate.

Application References
  1. Anusha Jayaraman, et al. 17?-Estradiol and Progesterone Regulate Expression of ?-Amyloid Clearance Factors in Primary Neuron Cultures and Female Rat Brain Endocrinology, 153: 5467 - 5479, Nov 2012.
  2. Thakker DR, et al. Intracerebroventricular amyloid-beta antibodies reduce cerebral amyloid angiopathy and associated micro-hemorrhages in aged Tg2576 mice. Proc Natl Acad Sci USA Feb 25, 2009.
Product Citations
  1. Wang A, et al. 2016. Proc Natl Acad Sci U S A. 113: 15120 - 15125. PubMed
  2. Shapira R,et al. 2017. Neurobiol Aging.. 10.1016/j.neurobiolaging.2017.10.007. PubMed
Publication Library
Standard Range
7.4 - 250 pg/mL

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Gene ID
351 View all products for this Gene ID
View information about beta-Amyloid on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 4    Revision Date: 03-21-2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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