KIRAVIA Blue 520™ anti-human CD199 (CCR9) Antibody

Pricing & Availability
Clone
L053E8 (See other available formats)
Regulatory Status
RUO
Other Names
GPR 9-6, GPR28, CDw199, Chemokine Receptor 9
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
L053E8_KB520_CD199_CCR9_Antibody_042121.png
Human acute lymphoblastic leukemia cell line, MOLT-4, was stained with anti-human CD199 (CCR9) (clone L053E8) KIRAVIA Blue 520™ (filled histogram) or mouse IgG2a, κ KIRAVIA Blue 520™ isotype control (open histogram).
  • L053E8_KB520_CD199_CCR9_Antibody_042121.png
    Human acute lymphoblastic leukemia cell line, MOLT-4, was stained with anti-human CD199 (CCR9) (clone L053E8) KIRAVIA Blue 520™ (filled histogram) or mouse IgG2a, κ KIRAVIA Blue 520™ isotype control (open histogram).
See KIRAVIA Blue 520™ spectral data
Cat # Size Price Quantity Avail. Save
358925 25 tests 100€
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358926 100 tests 236€
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Description

Human CD199, also known as CCR9, is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in the gut-associated immune response. It is highly expressed on different stages of thymocytes and upregulated on CD4+ CD8+ cells. Expression of CCR9 on γ/δ T cells in the intraepithelial and small intestine has been reported. The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and the generation of gut-specific immunological memory.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cells transfected with human CCR9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm.


KIRAVIA Blue™ 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Sony and the Sony logo are registered trademarks of Sony Corporation.
Excitation Laser
Blue Laser (488 nm)

Antigen Details

Structure
A member of G-protein coupled receptors which contains seven transmembrane domains. Human CCR9 has above 80% amino acid sequence homology with its mouse counterpart.
Distribution

CD4+CD8+ thymocytes, γ/δ T cells, subset of memory T cells

Function
May direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory; co-receptor for HIV-1
Ligand/Receptor
CCL25
Cell Type
T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Zaballos A, et al. 1999. J. Immunol. 162:5671.
2. Wurbel MA, et al. 2007. J. Immunol. 178:7598.
3. Wurbel MA, et al. 2006. Eur. J. Immunol. 36:73.

Gene ID
10803 View all products for this Gene ID
UniProt
View information about CD199 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 04-22-2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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