Loading...

HRP anti-RBFOX1 (A2BP1) Antibody

Pricing & Availability
Clone
A18069B (See other available formats)
Regulatory Status
RUO
Other Names
RBFox1, A2BP1, RNA Binding fox-1 Homolog 1, Hexaribonucleotide Binding Protein 1, FOX1
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
A18069B_HRP_RBFOX1_Antibody_1_100219
Western blot of HRP anti-RBFOX1 (A2BP1) antibody (clone A18069B). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate.The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A18069B_HRP_RBFOX1_Antibody_1_100219
    Western blot of HRP anti-RBFOX1 (A2BP1) antibody (clone A18069B). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate.The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A18069B_HRP_RBFOX1_Antibody_2_100219
    Western blot of HRP anti-RBFOX1 (A2BP1) antibody (clone A18069B). Lane 1: Molecular weight marker; Lane 2: 10 µg of rat brain lysate; Lane 3: 10 µg of mouse brain Lysate. The blot was incubated with 0.01 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A18069B_HRP_RBFOX1_Antibody_3_100219
    IHC staining of HRP anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A18069B_HRP_RBFOX1_Antibody_4_100219
    IHC staining of HRP anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Compare all formats
Cat # Size Price Quantity Check Availability Save
862703 25 µg 113€
 
Check Availability


Need larger quantities of this item?
Request Bulk Quote

This item is currently unavailable
 
862704 100 µg 249€
 
Check Availability


Need larger quantities of this item?
Request Bulk Quote

This item is currently unavailable
 
This product is scheduled for discontinuation.
Description

Rbfox1 is a member of the RNA binding protein family that shares a conservative RNA recognition motif. Rbfox1 has been shown to modulate alternative splicing by binding the consensus RNA sequence motif (U)GCAUG within introns. The interaction between cytoplasmic Rbfox1 and mRNA affects mRNA stability and translation. The binding of Rbfox1 protein to the C-terminus of ataxin-2 may be a contributing factor to the development of spinocerebellar ataxia type 2 .

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, a concentration range of 0.02 - 2.0 µg/ml for human and 0.01 - 0.02 µg/ml for murine is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2820145 (BioLegend Cat. No. 862703)
AB_2820146 (BioLegend Cat. No. 862704)

Antigen Details

Structure
RBFOX1 is a 416 amino acid protein with an apparent molecular mass of ~ 45 kD.
Distribution

Tissue Distribution: brain and heart.
Cellular Distribution: nucleus, golgi apparatus, and cytosol.

Function
RBFOX1 is an RNA binding protein that modulates mRNA alternative splicing.
Interaction
RNA, ataxin-2
Cell Type
Neurons, Oligodendrocytes
Biology Area
Neurodegeneration, Protein Synthesis, Transcription Factors
Antigen References
  1. Wang ET, et al. 2016. J. Neurosci. 36(45):11418
  2. Duan W, et al. 2016. Mol Neurobiol. 53(3):1637
  3. Kuroyanagi H, 2009. Cell Mol Life Sci. 66(24):3895
Gene ID
54715 View all products for this Gene ID 268859 View all products for this Gene ID 302920 View all products for this Gene ID
UniProt
View information about RBFOX1 on UniProt.org
Go To Top Version: 2    Revision Date: 01-20-2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account